Regulation of equine herpesvirus type 1 gene expression: Characterization of immediate early, early, and late transcription

Wayne L. Gray, Raymond P. Baumann, Alice T. Robertson, Gretchen B. Caughman, Dennis J. O'Callaghan, John Staczek

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.0). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of viral transcripts in oncogenically transformed and persistently infected cells.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalVirology
Volume158
Issue number1
DOIs
StatePublished - May 1987

ASJC Scopus subject areas

  • Virology

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