Regulation of expression of the endothelial cell nitric oxide synthase

David G. Harrison, Hassan Sayegh, Yuichi Ohara, Nobutaka Inoue, Richard C. Venema

Research output: Contribution to journalArticle

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Abstract

Recent studies have provided insight into how the expression of endothelial cell nitric oxide synthase (ecNOS) is regulated. The promoter of ecNOS has several features that are compatible with a constitutively expressed, so-called 'house keeping' gene. These include absence of a TATA box and the presence of Sp1 binding sites located near the transcription start site. The promoter also contains a number of putative binding domains which suggests that it may be regulated by a variety of transcription factor mediated signals. Studies of cultured endothelial cells suggest that ecNOS expression is modulated by shear stress, transforming growth factor beta, inhibition of protein kinase C and the state of proliferation. These experiments indicate that although the ecNOS is a 'constitutively expressed' gene, its content in the endothelium is subject to modest degrees of regulation that may have important physiological and pathophysiological implications.

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalClinical and Experimental Pharmacology and Physiology
Volume23
Issue number3
DOIs
Publication statusPublished - Jan 1 1996

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Keywords

  • Sp1
  • growth
  • shear stress
  • transforming growth factor beta

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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