Regulation of ganglioside biosynthesis in the nervous system

Robert K Yu, Erhard Bieberich, Tian Xia, Guichao Zeng

Research output: Contribution to journalReview article

127 Citations (Scopus)

Abstract

Ganglioside biosynthesis is strictly regulated by the activities of glycosyltransferases and is necessarily controlled at the levels of gene transcription and posttranslational modification. Cells can switch between expressing simple and complex gangliosides or between different series within these two groups during brain development. The sequential biosynthesis of gangliosides in parallel enzymatic pathways, however, requires fine-tuned subcellular sequestration and orchestration of glycosyltransferases. A popular model predicts that this regulation is achieved by the vectorial organization of ganglioside biosynthesis: sequential biosynthetic steps occur with the traffic of ganglioside intermediates through subsequent subcellular compartments. Here, we review current models for the subcellular distribution of glycosyltransferases and discuss results that suggest a critical role of N-glycosylation for the processing, transport, and complex formation of these enzymes. In this context, we attempt to illustrate the regulation of ganglioside biosynthesis as well as the biological significance of N-glycosylation as a posttranslational regulatory mechanism. We also review the results of analyses of the 5′ regulatory sequences of several glycosyltransferases in ganglioside biosynthesis and provide insights into how their synthesis can be regulated at the level of transcription.

Original languageEnglish (US)
Pages (from-to)783-793
Number of pages11
JournalJournal of Lipid Research
Volume45
Issue number5
DOIs
StatePublished - Jan 1 2004

Fingerprint

Gangliosides
Biosynthesis
Neurology
Nervous System
Glycosyltransferases
Glycosylation
Transcription
Post Translational Protein Processing
Brain
Genes
Switches
Enzymes
Processing

Keywords

  • Ceramide
  • Endoplasmic reticulum
  • Glycosyltransferase
  • Golgi
  • N-glycosylation
  • Posttranslational modification
  • Transcription
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Regulation of ganglioside biosynthesis in the nervous system. / Yu, Robert K; Bieberich, Erhard; Xia, Tian; Zeng, Guichao.

In: Journal of Lipid Research, Vol. 45, No. 5, 01.01.2004, p. 783-793.

Research output: Contribution to journalReview article

Yu, Robert K ; Bieberich, Erhard ; Xia, Tian ; Zeng, Guichao. / Regulation of ganglioside biosynthesis in the nervous system. In: Journal of Lipid Research. 2004 ; Vol. 45, No. 5. pp. 783-793.
@article{4ce0f4515a0d4f52a794473bbd9debb2,
title = "Regulation of ganglioside biosynthesis in the nervous system",
abstract = "Ganglioside biosynthesis is strictly regulated by the activities of glycosyltransferases and is necessarily controlled at the levels of gene transcription and posttranslational modification. Cells can switch between expressing simple and complex gangliosides or between different series within these two groups during brain development. The sequential biosynthesis of gangliosides in parallel enzymatic pathways, however, requires fine-tuned subcellular sequestration and orchestration of glycosyltransferases. A popular model predicts that this regulation is achieved by the vectorial organization of ganglioside biosynthesis: sequential biosynthetic steps occur with the traffic of ganglioside intermediates through subsequent subcellular compartments. Here, we review current models for the subcellular distribution of glycosyltransferases and discuss results that suggest a critical role of N-glycosylation for the processing, transport, and complex formation of these enzymes. In this context, we attempt to illustrate the regulation of ganglioside biosynthesis as well as the biological significance of N-glycosylation as a posttranslational regulatory mechanism. We also review the results of analyses of the 5′ regulatory sequences of several glycosyltransferases in ganglioside biosynthesis and provide insights into how their synthesis can be regulated at the level of transcription.",
keywords = "Ceramide, Endoplasmic reticulum, Glycosyltransferase, Golgi, N-glycosylation, Posttranslational modification, Transcription, Transcription factor",
author = "Yu, {Robert K} and Erhard Bieberich and Tian Xia and Guichao Zeng",
year = "2004",
month = "1",
day = "1",
doi = "10.1194/jlr.R300020-JLR200",
language = "English (US)",
volume = "45",
pages = "783--793",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - Regulation of ganglioside biosynthesis in the nervous system

AU - Yu, Robert K

AU - Bieberich, Erhard

AU - Xia, Tian

AU - Zeng, Guichao

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Ganglioside biosynthesis is strictly regulated by the activities of glycosyltransferases and is necessarily controlled at the levels of gene transcription and posttranslational modification. Cells can switch between expressing simple and complex gangliosides or between different series within these two groups during brain development. The sequential biosynthesis of gangliosides in parallel enzymatic pathways, however, requires fine-tuned subcellular sequestration and orchestration of glycosyltransferases. A popular model predicts that this regulation is achieved by the vectorial organization of ganglioside biosynthesis: sequential biosynthetic steps occur with the traffic of ganglioside intermediates through subsequent subcellular compartments. Here, we review current models for the subcellular distribution of glycosyltransferases and discuss results that suggest a critical role of N-glycosylation for the processing, transport, and complex formation of these enzymes. In this context, we attempt to illustrate the regulation of ganglioside biosynthesis as well as the biological significance of N-glycosylation as a posttranslational regulatory mechanism. We also review the results of analyses of the 5′ regulatory sequences of several glycosyltransferases in ganglioside biosynthesis and provide insights into how their synthesis can be regulated at the level of transcription.

AB - Ganglioside biosynthesis is strictly regulated by the activities of glycosyltransferases and is necessarily controlled at the levels of gene transcription and posttranslational modification. Cells can switch between expressing simple and complex gangliosides or between different series within these two groups during brain development. The sequential biosynthesis of gangliosides in parallel enzymatic pathways, however, requires fine-tuned subcellular sequestration and orchestration of glycosyltransferases. A popular model predicts that this regulation is achieved by the vectorial organization of ganglioside biosynthesis: sequential biosynthetic steps occur with the traffic of ganglioside intermediates through subsequent subcellular compartments. Here, we review current models for the subcellular distribution of glycosyltransferases and discuss results that suggest a critical role of N-glycosylation for the processing, transport, and complex formation of these enzymes. In this context, we attempt to illustrate the regulation of ganglioside biosynthesis as well as the biological significance of N-glycosylation as a posttranslational regulatory mechanism. We also review the results of analyses of the 5′ regulatory sequences of several glycosyltransferases in ganglioside biosynthesis and provide insights into how their synthesis can be regulated at the level of transcription.

KW - Ceramide

KW - Endoplasmic reticulum

KW - Glycosyltransferase

KW - Golgi

KW - N-glycosylation

KW - Posttranslational modification

KW - Transcription

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=2142655789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2142655789&partnerID=8YFLogxK

U2 - 10.1194/jlr.R300020-JLR200

DO - 10.1194/jlr.R300020-JLR200

M3 - Review article

C2 - 15087476

AN - SCOPUS:2142655789

VL - 45

SP - 783

EP - 793

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 5

ER -