Regulation of glycolipid synthesis in HL-60 cells by antisense oligodeoxynucleotides to glycosyltransferase sequences: Effect on cellular differentiation

Guichao Zeng, Toshio Ariga, Xin Bin Gu, Robert K. Yu

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Treatment of the human promyelocytic leukemia cell line HL-60 with antisense oligodeoxynucleotides to UDP-N-acetylgalactosamine:β-1,4-N- acetylgalactosaminyl-transferase (GM2-synthase; EC 2.4.1.92) and CMP-sialic acid:α-2,8-sialyltransferase (GD3-synthase; EC 2.4.99.8) sequences effectively down-regulated the synthesis of more complex gangliosides in the ganglioside synthetic pathways after GM3, resulting in a remarkable increase in endogenous GM3 with concomitant decreases in more complex gangliosides. The treated cells underwent monocytic differentiation as judged by morphological changes, adherent ability, and nitroblue tetrazolium staining. These data provide evidence that the increased endogenous ganglioside GM3 may play an important role in regulating cellular differentiation and that the antisense DNA technique proves to be a powerful tool in manipulating glycolipid synthesis in the cell.

Original languageEnglish (US)
Pages (from-to)8670-8674
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number19
DOIs
StatePublished - Sep 12 1995

Fingerprint

(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
Glycosyltransferases
Gangliosides
Oligodeoxyribonucleotides
HL-60 Cells
Glycolipids
Uridine Diphosphate N-Acetylgalactosamine
Cytidine Monophosphate N-Acetylneuraminic Acid
Antisense DNA
G(M3) Ganglioside
Sialyltransferases
Nitroblue Tetrazolium
Transferases
Leukemia
Staining and Labeling
Cell Line

Keywords

  • cell maturation
  • gangliosides
  • gene expression

ASJC Scopus subject areas

  • General

Cite this

@article{d4eb720b151c49329c0bbee101a711e0,
title = "Regulation of glycolipid synthesis in HL-60 cells by antisense oligodeoxynucleotides to glycosyltransferase sequences: Effect on cellular differentiation",
abstract = "Treatment of the human promyelocytic leukemia cell line HL-60 with antisense oligodeoxynucleotides to UDP-N-acetylgalactosamine:β-1,4-N- acetylgalactosaminyl-transferase (GM2-synthase; EC 2.4.1.92) and CMP-sialic acid:α-2,8-sialyltransferase (GD3-synthase; EC 2.4.99.8) sequences effectively down-regulated the synthesis of more complex gangliosides in the ganglioside synthetic pathways after GM3, resulting in a remarkable increase in endogenous GM3 with concomitant decreases in more complex gangliosides. The treated cells underwent monocytic differentiation as judged by morphological changes, adherent ability, and nitroblue tetrazolium staining. These data provide evidence that the increased endogenous ganglioside GM3 may play an important role in regulating cellular differentiation and that the antisense DNA technique proves to be a powerful tool in manipulating glycolipid synthesis in the cell.",
keywords = "cell maturation, gangliosides, gene expression",
author = "Guichao Zeng and Toshio Ariga and Gu, {Xin Bin} and Yu, {Robert K.}",
year = "1995",
month = "9",
day = "12",
doi = "10.1073/pnas.92.19.8670",
language = "English (US)",
volume = "92",
pages = "8670--8674",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "19",

}

TY - JOUR

T1 - Regulation of glycolipid synthesis in HL-60 cells by antisense oligodeoxynucleotides to glycosyltransferase sequences

T2 - Effect on cellular differentiation

AU - Zeng, Guichao

AU - Ariga, Toshio

AU - Gu, Xin Bin

AU - Yu, Robert K.

PY - 1995/9/12

Y1 - 1995/9/12

N2 - Treatment of the human promyelocytic leukemia cell line HL-60 with antisense oligodeoxynucleotides to UDP-N-acetylgalactosamine:β-1,4-N- acetylgalactosaminyl-transferase (GM2-synthase; EC 2.4.1.92) and CMP-sialic acid:α-2,8-sialyltransferase (GD3-synthase; EC 2.4.99.8) sequences effectively down-regulated the synthesis of more complex gangliosides in the ganglioside synthetic pathways after GM3, resulting in a remarkable increase in endogenous GM3 with concomitant decreases in more complex gangliosides. The treated cells underwent monocytic differentiation as judged by morphological changes, adherent ability, and nitroblue tetrazolium staining. These data provide evidence that the increased endogenous ganglioside GM3 may play an important role in regulating cellular differentiation and that the antisense DNA technique proves to be a powerful tool in manipulating glycolipid synthesis in the cell.

AB - Treatment of the human promyelocytic leukemia cell line HL-60 with antisense oligodeoxynucleotides to UDP-N-acetylgalactosamine:β-1,4-N- acetylgalactosaminyl-transferase (GM2-synthase; EC 2.4.1.92) and CMP-sialic acid:α-2,8-sialyltransferase (GD3-synthase; EC 2.4.99.8) sequences effectively down-regulated the synthesis of more complex gangliosides in the ganglioside synthetic pathways after GM3, resulting in a remarkable increase in endogenous GM3 with concomitant decreases in more complex gangliosides. The treated cells underwent monocytic differentiation as judged by morphological changes, adherent ability, and nitroblue tetrazolium staining. These data provide evidence that the increased endogenous ganglioside GM3 may play an important role in regulating cellular differentiation and that the antisense DNA technique proves to be a powerful tool in manipulating glycolipid synthesis in the cell.

KW - cell maturation

KW - gangliosides

KW - gene expression

UR - http://www.scopus.com/inward/record.url?scp=0029073388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029073388&partnerID=8YFLogxK

U2 - 10.1073/pnas.92.19.8670

DO - 10.1073/pnas.92.19.8670

M3 - Article

C2 - 7567994

AN - SCOPUS:0029073388

VL - 92

SP - 8670

EP - 8674

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 19

ER -