Regulation of MuSK expression by a novel signaling pathway

Chang Hoon Kim, Wen C. Xiong, Lin Mei

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

MuSK is a receptor tyrosine kinase essential for neuromuscular junction formation. Expression of the MuSK gene is tightly regulated during development and at the neuromuscular junction. However, little is known about molecular mechanisms regulating its gene expression. Here we report a characterization of the promoter of the mouse MuSK gene. The transcription of MuSK starts at multiple sites with a major site 51 nt upstream of the translation start site. We have identified an E-box-like cis-element that is both required and sufficient for differentiation-dependent transcription. Interestingly, the promoter activity of the MuSK gene did not respond to neuregulin, a factor believed to mediate the synapse-specific transcription of acetylcholine receptor subunit genes. Rather, MuSK expression is increased in muscle cells stimulated with Wnt or at conditions when the Wnt signaling was activated. These results suggest a novel mechanism for the MuSK synapse-specific expression.

Original languageEnglish (US)
Pages (from-to)38522-38527
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number40
DOIs
StatePublished - Oct 3 2003

Fingerprint

Transcription
Genes
Neuromuscular Junction
Synapses
Neuregulins
Gene Expression
Receptor Protein-Tyrosine Kinases
Cholinergic Receptors
Muscle Cells
Gene expression
Muscle
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Regulation of MuSK expression by a novel signaling pathway. / Kim, Chang Hoon; Xiong, Wen C.; Mei, Lin.

In: Journal of Biological Chemistry, Vol. 278, No. 40, 03.10.2003, p. 38522-38527.

Research output: Contribution to journalArticle

Kim, Chang Hoon ; Xiong, Wen C. ; Mei, Lin. / Regulation of MuSK expression by a novel signaling pathway. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 40. pp. 38522-38527.
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