Regulation of neural progenitor cell motility by ceramide and potential implications for mouse brain development

Guanghu Wang, Kannan Krishnamurthy, Ying Wei Chiang, Somsankar Dasgupta, Erhard Bieberich

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We provide evidence that the sphingolipid ceramide, in addition to its pro-apoptotic function, regulates neural progenitor (NP) motility in vitro and brain development in vivo. Ceramide (N-palmitoyl D-erythro sphingosine and N-oleoyl D-erythro sphingosine) and the ceramide analog N-oleoyl serinol (S18) stimulate migration of NPs in scratch (wounding) migration assays. Sphingolipid depletion by inhibition of de novo ceramide biosynthesis, or ceramide inactivation using an anti - ceramide antibody, obliterates NP motility, which is restored by ceramide or S18. These results suggest that ceramide is crucial for NP motility. Wounding of the NP monolayer activates neutral sphingomyelinase indicating that ceramide is generated from sphingomyelin. In membrane processes, ceramide is co-distributed with its binding partner atypical protein kinase C ζ/λ (aPKC), and Cdc42, α/β-tubulin, and p-catenin, three proteins involved in aPKC-dependent regulation of cell polarity and motility. Sphingolipid depletion by myriocin prevents membrane translocation of aPKC and Cdc42, which is restored by ceramide or S18. These results suggest that ceramide-mediated membrane association of aPKC/Cdc42 is important for NP motility. In vivo, sphingolipid depletion leads to ectopic localization of mitotic or post-mitotic neural cells in the embryonic brain, while S18 restores the normal brain organization. In summary, our study provides novel evidence that ceramide is critical for NP motility and polarity in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)718-733
Number of pages16
JournalJournal of Neurochemistry
Volume106
Issue number2
DOIs
StatePublished - Jul 1 2008

Fingerprint

Ceramides
Cell Movement
Brain
Stem Cells
Sphingolipids
Membranes
Sphingomyelin Phosphodiesterase
Catenins
Cell Polarity
Sphingomyelins
Biosynthesis
Tubulin
Anti-Idiotypic Antibodies
Monolayers
Assays

Keywords

  • Brain morphogenesis
  • Ceramide
  • Motility
  • Polarity
  • Sphingolipids

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Regulation of neural progenitor cell motility by ceramide and potential implications for mouse brain development. / Wang, Guanghu; Krishnamurthy, Kannan; Chiang, Ying Wei; Dasgupta, Somsankar; Bieberich, Erhard.

In: Journal of Neurochemistry, Vol. 106, No. 2, 01.07.2008, p. 718-733.

Research output: Contribution to journalArticle

Wang, Guanghu ; Krishnamurthy, Kannan ; Chiang, Ying Wei ; Dasgupta, Somsankar ; Bieberich, Erhard. / Regulation of neural progenitor cell motility by ceramide and potential implications for mouse brain development. In: Journal of Neurochemistry. 2008 ; Vol. 106, No. 2. pp. 718-733.
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