Regulation of neural stem cell proliferation and differentiation by Kinesin family member 2a

Dong Sun, Xue Zhou, Hua Li Yu, Xiao Xiao He, Wei Xiang Guo, Wen Cheng Xiong, Xiao Juan Zhu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In the developing neocortex, cells in the ventricular/subventricular zone are largely multipotent neural stem cells and neural progenitor cells. These cells undergo self-renewal at the early stage of embryonic development to amplify the progenitor pool and subsequently differentiate into neurons. It is thus of considerable interest to investigate mechanisms controlling the switch from neural stem cells or neural progenitor cells to neurons. Here, we present evidence that Kif2a, a member of the Kinesin-13 family, plays a role in regulating the proliferation and differentiation of neural stem cells or neural progenitor cells at embryonic day 13.5. Silencing Kif2a by use of in utero electroporation of Kif2a shRNA reduced neural stem cells proliferation or self-renewal but increased neuronal differentiation. We further found that knockdown of Kif2a decreased the protein level of β-catenin, which is a critical molecule for neocortical neurogenesis. Together, these results reveal an important function of Kif2a in embryonic neocortical neurogenesis.

Original languageEnglish (US)
Article numbere0179047
JournalPloS one
Volume12
Issue number6
DOIs
StatePublished - Jun 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Sun, D., Zhou, X., Yu, H. L., He, X. X., Guo, W. X., Xiong, W. C., & Zhu, X. J. (2017). Regulation of neural stem cell proliferation and differentiation by Kinesin family member 2a. PloS one, 12(6), [e0179047]. https://doi.org/10.1371/journal.pone.0179047