Regulation of primary cilia formation by ceramide

Guanghu Wang, Kannan Krishnamurthy, Erhard Bieberich

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The primary cilium is an important sensory organelle, the regulation of which is not fully understood. We found that in polarized Madin-Darby Canine Kidney cells, the sphingolipid ceramide is specifically distributed to a cis-Golgi compartment at the base of the primary cilium. This compartment immunostained for the centrosome marker γ-tubulin, the Rho type GTPase cell division cycle 42 (Cdc42), and atypical protein kinase Cζ/λ (aPKC), a kinase activated by ceramide and associated with a polarity protein complex consisting of partitioning defective (Par)6 and Cdc42. Inhibition of ceramide biosynthesis with Fumonisin B1 prevented codistribution of aPKC and Cdc42 in the centrosomal/pericentriolar compartment and severely impaired ciliogenesis. Cilium formation and codistribution of aPKC and Cdc42 were restored by incubation with N-acetyl or N-palmitoyl sphingosine (C2 or C16 ceramide), or the ceramide analog N-oleoyl serinol (S18). Cilium formation was also restored by the glycogen synthase kinase-3β (GSK-3β) inhibitor indirubin-3-monoxime, suggesting that regulation of ciliogenesis depends on the inhibition of GSK-3β by ceramide-activated aPKC. Consistently, inhibition of aPKC with a pseudosubstrate inhibitor prevented restoration of ciliogenesis by C2 ceramide or S18. Our data show for the first time ceramide is required for primary cilium formation.

Original languageEnglish (US)
Pages (from-to)2103-2110
Number of pages8
JournalJournal of Lipid Research
Volume50
Issue number10
DOIs
StatePublished - Nov 2 2009

Fingerprint

Cilia
Ceramides
Cell Cycle
Glycogen Synthase Kinase 3
rho GTP-Binding Proteins
Centrosome
Sphingolipids
Madin Darby Canine Kidney Cells
Sphingosine
Biosynthesis
Tubulin
Organelles
Restoration
Phosphotransferases
Cells
PKC-3 protein
Proteins

Keywords

  • Cell polarity
  • Centrosome
  • Golgi
  • Primary cilium
  • Sphingolipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Regulation of primary cilia formation by ceramide. / Wang, Guanghu; Krishnamurthy, Kannan; Bieberich, Erhard.

In: Journal of Lipid Research, Vol. 50, No. 10, 02.11.2009, p. 2103-2110.

Research output: Contribution to journalArticle

Wang, G, Krishnamurthy, K & Bieberich, E 2009, 'Regulation of primary cilia formation by ceramide', Journal of Lipid Research, vol. 50, no. 10, pp. 2103-2110. https://doi.org/10.1194/jlr.M900097-JLR200
Wang, Guanghu ; Krishnamurthy, Kannan ; Bieberich, Erhard. / Regulation of primary cilia formation by ceramide. In: Journal of Lipid Research. 2009 ; Vol. 50, No. 10. pp. 2103-2110.
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