Regulation of T-cell function by antibodies

Enhancement of the response of human T-cell clones to hepatitis B surface antigen by antigen-specific monoclonal antibodies

Esteban Celis, V. R. Zurawski, Wen Chang Tse Wen Chang

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42 Citations (Scopus)

Abstract

Eight mouse monoclonal antibodies specific for hepatitis B surface antigen (HBsAg) were examined for their effects on the antigen-induced proliferative response and lymphokine production of human HBsAg-specific T-cell clones in vitro. While all specifically enhanced the T-cell proliferative response, antibodies of the IgG class were generally more effective than those of the IgM class. Both the divalent F(ab')2 and the monovalent Fab fragments of an IgG monoclonal antibody had no effects, indicating that the Fc portion of the antibody molecules was required. Since antigen-presenting cells bear surface receptors for the Fc of IgGs and fewer or none for that of IgMs, the above results also suggest that antibodies enhance the capture of antigens by antigen-presenting cells as a result of the binding of antigen-antibody complexes to the Fc receptors on these cells. In addition to potentiating the proliferation of the T-cell clones, antibodies also increased the antigen-induced production of interferon-γ by these cells. The present in vitro studies suggest that antibodies may regulate immune responses and do so by enhancing antigen presentation and thus augmenting antigen-induced activation and clonal expansion of T cells.

Original languageEnglish (US)
Pages (from-to)6846-6850
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number21 I
DOIs
StatePublished - Jan 1 1984

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Hepatitis B Surface Antigens
Antibody Formation
Clone Cells
Monoclonal Antibodies
T-Lymphocytes
Antigens
Antibodies
Antigen-Presenting Cells
Immunoglobulin G
Immunoglobulin Fab Fragments
Fc Receptors
Immunoglobulin Isotypes
Lymphokines
Antigen Presentation
Cell Surface Receptors
Antigen-Antibody Complex
Interferons
Immunoglobulin M
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

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abstract = "Eight mouse monoclonal antibodies specific for hepatitis B surface antigen (HBsAg) were examined for their effects on the antigen-induced proliferative response and lymphokine production of human HBsAg-specific T-cell clones in vitro. While all specifically enhanced the T-cell proliferative response, antibodies of the IgG class were generally more effective than those of the IgM class. Both the divalent F(ab')2 and the monovalent Fab fragments of an IgG monoclonal antibody had no effects, indicating that the Fc portion of the antibody molecules was required. Since antigen-presenting cells bear surface receptors for the Fc of IgGs and fewer or none for that of IgMs, the above results also suggest that antibodies enhance the capture of antigens by antigen-presenting cells as a result of the binding of antigen-antibody complexes to the Fc receptors on these cells. In addition to potentiating the proliferation of the T-cell clones, antibodies also increased the antigen-induced production of interferon-γ by these cells. The present in vitro studies suggest that antibodies may regulate immune responses and do so by enhancing antigen presentation and thus augmenting antigen-induced activation and clonal expansion of T cells.",
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