Regulation of the human immune response to HBsAg: Effects of antibodies and antigen conformation in the stimulation of helper T cells by HBsAg

Esteban Celis, Ikunoshin Kato, Richard W. Miller, Tse Wen Chang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The role of accessory cells (antigen‐presenting cells) in binding HBsAg in the response of human T cells to this antigen was studied. Antibodies to HBsAg of IgG class increased significantly the amount of HBsAg that was captured and internalized by accessory cells in vitro. On the other hand, antibodies to HBsAg of IgM class or the F(ab‐)2 and Fab fragments of antibodies to HBsAg of IgG class did not modify the amount of HBsAg associated to these cells. HBsAg that was subjected to various denaturing treatments (acid, organic solvents, urea and heat) was compared for its capacity to react with antibody to HBsAg and stimulate the response of helper T lymphocytes. Results presented here indicate that HBsAg denatured by treatment with formic acid was captured by accessory cells and presented to the T cells much more efficiently than the native HBsAg. These results suggest that the response of helper T lymphocytes to some antigens such as HBsAg can be affected greatly by the presence of antibodies or the antigens‐ conformation.

Original languageEnglish (US)
Pages (from-to)744-751
Number of pages8
JournalHepatology
Volume5
Issue number5
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Hepatitis B Surface Antigens
Helper-Inducer T-Lymphocytes
Antigens
Antibodies
formic acid
Immunoglobulin G
T-Lymphocytes
Immunoglobulin Fab Fragments
Immunoglobulin M
Urea
Hot Temperature
Acids

ASJC Scopus subject areas

  • Hepatology

Cite this

Regulation of the human immune response to HBsAg : Effects of antibodies and antigen conformation in the stimulation of helper T cells by HBsAg. / Celis, Esteban; Kato, Ikunoshin; Miller, Richard W.; Chang, Tse Wen.

In: Hepatology, Vol. 5, No. 5, 01.01.1985, p. 744-751.

Research output: Contribution to journalArticle

@article{aeb0ace67d674fc1946e314ecf3258ed,
title = "Regulation of the human immune response to HBsAg: Effects of antibodies and antigen conformation in the stimulation of helper T cells by HBsAg",
abstract = "The role of accessory cells (antigen‐presenting cells) in binding HBsAg in the response of human T cells to this antigen was studied. Antibodies to HBsAg of IgG class increased significantly the amount of HBsAg that was captured and internalized by accessory cells in vitro. On the other hand, antibodies to HBsAg of IgM class or the F(ab‐)2 and Fab fragments of antibodies to HBsAg of IgG class did not modify the amount of HBsAg associated to these cells. HBsAg that was subjected to various denaturing treatments (acid, organic solvents, urea and heat) was compared for its capacity to react with antibody to HBsAg and stimulate the response of helper T lymphocytes. Results presented here indicate that HBsAg denatured by treatment with formic acid was captured by accessory cells and presented to the T cells much more efficiently than the native HBsAg. These results suggest that the response of helper T lymphocytes to some antigens such as HBsAg can be affected greatly by the presence of antibodies or the antigens‐ conformation.",
author = "Esteban Celis and Ikunoshin Kato and Miller, {Richard W.} and Chang, {Tse Wen}",
year = "1985",
month = "1",
day = "1",
doi = "10.1002/hep.1840050507",
language = "English (US)",
volume = "5",
pages = "744--751",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Regulation of the human immune response to HBsAg

T2 - Effects of antibodies and antigen conformation in the stimulation of helper T cells by HBsAg

AU - Celis, Esteban

AU - Kato, Ikunoshin

AU - Miller, Richard W.

AU - Chang, Tse Wen

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The role of accessory cells (antigen‐presenting cells) in binding HBsAg in the response of human T cells to this antigen was studied. Antibodies to HBsAg of IgG class increased significantly the amount of HBsAg that was captured and internalized by accessory cells in vitro. On the other hand, antibodies to HBsAg of IgM class or the F(ab‐)2 and Fab fragments of antibodies to HBsAg of IgG class did not modify the amount of HBsAg associated to these cells. HBsAg that was subjected to various denaturing treatments (acid, organic solvents, urea and heat) was compared for its capacity to react with antibody to HBsAg and stimulate the response of helper T lymphocytes. Results presented here indicate that HBsAg denatured by treatment with formic acid was captured by accessory cells and presented to the T cells much more efficiently than the native HBsAg. These results suggest that the response of helper T lymphocytes to some antigens such as HBsAg can be affected greatly by the presence of antibodies or the antigens‐ conformation.

AB - The role of accessory cells (antigen‐presenting cells) in binding HBsAg in the response of human T cells to this antigen was studied. Antibodies to HBsAg of IgG class increased significantly the amount of HBsAg that was captured and internalized by accessory cells in vitro. On the other hand, antibodies to HBsAg of IgM class or the F(ab‐)2 and Fab fragments of antibodies to HBsAg of IgG class did not modify the amount of HBsAg associated to these cells. HBsAg that was subjected to various denaturing treatments (acid, organic solvents, urea and heat) was compared for its capacity to react with antibody to HBsAg and stimulate the response of helper T lymphocytes. Results presented here indicate that HBsAg denatured by treatment with formic acid was captured by accessory cells and presented to the T cells much more efficiently than the native HBsAg. These results suggest that the response of helper T lymphocytes to some antigens such as HBsAg can be affected greatly by the presence of antibodies or the antigens‐ conformation.

UR - http://www.scopus.com/inward/record.url?scp=0021934352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021934352&partnerID=8YFLogxK

U2 - 10.1002/hep.1840050507

DO - 10.1002/hep.1840050507

M3 - Article

C2 - 3161815

AN - SCOPUS:0021934352

VL - 5

SP - 744

EP - 751

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 5

ER -