TY - CHAP
T1 - Regulation of the Trafficking and Function of G Protein-Coupled Receptors by Rab1 GTPase in Cardiomyocytes
AU - Wu, Guangyu
N1 - Funding Information:
This work was supported by the National Institutes of Health grants R01‐GM076167 and P20‐R018766. The author thanks Stephen M. Lanier, John D. Hildebrandt (Medical University of South Carolina), Kenneth P. Minneman, and Kenneth E. Bernstein (Emory University) for sharing reagents.
PY - 2008
Y1 - 2008
N2 - G protein-coupled receptors (GPCRs) play a crucial role in regulating cardiac growth and function under normal and diseased conditions. It has been well documented that the precise function of GPCRs is controlled by intracellular trafficking of the receptors. Compared with the extensive studies on the events of the endocytic pathway, molecular mechanism underlying the transport process of GPCRs from the endoplasmic reticulum (ER) through the Golgi to the cell surface and regulation of receptor signaling by these processes in cardiac myocytes remain poorly defined. This chapter describes the methods to characterize the function of Rab1 GTPase, which modulates protein transport from the ER to the Golgi apparatus, in the trafficking and signaling of angiotensin II type 1 receptor (AT1R), α1-adrenergic receptor (AR), and β-AR, and in hypertrophic growth in response to agonist stimulation in neonatal cardiac myocytes.
AB - G protein-coupled receptors (GPCRs) play a crucial role in regulating cardiac growth and function under normal and diseased conditions. It has been well documented that the precise function of GPCRs is controlled by intracellular trafficking of the receptors. Compared with the extensive studies on the events of the endocytic pathway, molecular mechanism underlying the transport process of GPCRs from the endoplasmic reticulum (ER) through the Golgi to the cell surface and regulation of receptor signaling by these processes in cardiac myocytes remain poorly defined. This chapter describes the methods to characterize the function of Rab1 GTPase, which modulates protein transport from the ER to the Golgi apparatus, in the trafficking and signaling of angiotensin II type 1 receptor (AT1R), α1-adrenergic receptor (AR), and β-AR, and in hypertrophic growth in response to agonist stimulation in neonatal cardiac myocytes.
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U2 - 10.1016/S0076-6879(07)38016-6
DO - 10.1016/S0076-6879(07)38016-6
M3 - Chapter
C2 - 18413252
AN - SCOPUS:45549107702
SN - 9780123739681
T3 - Methods in Enzymology
SP - 227
EP - 238
BT - Small GTPases in Disease, Part A
A2 - Balch, William
A2 - Hall, Alan
A2 - Der, Channing
ER -