Regulatory domain of human heat shock transcription factor-2 is not regulated by hemin or heat shock

Zhen Zhu, Nahid F Mivechi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Heat shock transcription factor 2 (HSF-2) activates transcription of heat shock proteins in response to hemin in the human erythroleukemia cell line, K562. To understand the regulation of HSF-2 activation, a series of deletion mutants of HSF-2 fused to the GAL-4 DNA binding domain were generated. We have found that human HSF-2 has a regulatory domain located in the carboxyl-terminal portion of the protein which represses the activity of its activation domain under normal physiological conditions. The repressive effects of this domain can be eliminated by its deletion in GAL4-HSF-2 fusion constructs. The regulatory domain of HSF-2 can also repress a heterologous chimeric activator that contains a portion of the VP16 activation domain. The activation domain of HSF-2 is a segment of approximately 77 amino acids located proximal to the carboxyl-terminal hydrophobic heptad repeat (leucine zipper 4) of the molecule. Interestingly, the GAL4-HSF-2 fusion protein and the 77 amino acids activation domain are inactive and are not activated by pretreatment of cells with either hemin or elevated temperature. Our data suggest that regulation of HSF-2 differs from HSF-1 in that its regulatory domain is not responsive to hemin or heat directly.

Original languageEnglish (US)
Pages (from-to)56-69
Number of pages14
JournalJournal of Cellular Biochemistry
Volume73
Issue number1
DOIs
StatePublished - Apr 1 1999

Fingerprint

Hemin
Shock
Hot Temperature
Chemical activation
Fusion reactions
Cells
Aminoacylation
heat shock transcription factor
human HSF2 protein
Amino Acids
Leucine Zippers
Leukemia, Erythroblastic, Acute
Transcription
Heat-Shock Proteins
Proteins
Cell Line
Molecules
Temperature
DNA

Keywords

  • HSF-2
  • Heat shock
  • Hemin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Regulatory domain of human heat shock transcription factor-2 is not regulated by hemin or heat shock. / Zhu, Zhen; Mivechi, Nahid F.

In: Journal of Cellular Biochemistry, Vol. 73, No. 1, 01.04.1999, p. 56-69.

Research output: Contribution to journalArticle

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