Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer

Xiongjie Jin, Jianjun Zhang, Yanning Gao, Keyue Ding, Naishu Wang, David Zhou, Jin Jen, Shujun Cheng

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Mitochondrial DNA (mtDNA) is known for its high frequencies of polymorphisms and mutations, some of which are related to various diseases, including cancers. However, roles of mutations and polymorphisms in some diseases are among heated debate, especially for cancer. To investigate the possible role of mtDNA mutations in lung cancer, we sequenced complete mtDNA of lung cancer tissues, corresponding normal (i.e., non-cancerous) lung tissues, and peripheral blood samples from 55 lung cancer patients and examined the relationship between mtDNA mutations or polymorphisms and clinical parameters. We identified 56 mutations in 33 (60%) of the 55 patients, including 48 point mutations, four single-nucleotide insertions, and four single-nucleotide deletions. Nineteen of these mutations resulted in amino acid substitution. These missense mtDNA mutations were distributed in 9 of 13 mitochondrial DNA coding genes. Three hundred eighty eight polymorphisms were identified among the 55 patients. Seventy-three polymorphisms resulted in amino acid substitution. There was no association of incidence of specific mtDNA mutation or polymorphism with patients' gender, age at diagnosis, smoking history, tumor type or tumor stage (P > 0.05). This study revealed a variety of mtDNA mutations and mtDNA polymorphisms in human lung cancer, some of which might be involved in human lung carcinogenesis.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalMitochondrion
Volume7
Issue number5
DOIs
StatePublished - Sep 1 2007

Fingerprint

Mitochondrial DNA
Lung Neoplasms
Mutation
Amino Acid Substitution
Neoplasms
Nucleotides
Lung
Mutation Rate
Point Mutation
Carcinogenesis
Smoking
History
Incidence
Genes

Keywords

  • Lung cancer
  • Mitochondrial DNA
  • Mutation

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer. / Jin, Xiongjie; Zhang, Jianjun; Gao, Yanning; Ding, Keyue; Wang, Naishu; Zhou, David; Jen, Jin; Cheng, Shujun.

In: Mitochondrion, Vol. 7, No. 5, 01.09.2007, p. 347-353.

Research output: Contribution to journalArticle

Jin, Xiongjie ; Zhang, Jianjun ; Gao, Yanning ; Ding, Keyue ; Wang, Naishu ; Zhou, David ; Jen, Jin ; Cheng, Shujun. / Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer. In: Mitochondrion. 2007 ; Vol. 7, No. 5. pp. 347-353.
@article{821b015f068d4d6e9d3e4f850bb03cc5,
title = "Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer",
abstract = "Mitochondrial DNA (mtDNA) is known for its high frequencies of polymorphisms and mutations, some of which are related to various diseases, including cancers. However, roles of mutations and polymorphisms in some diseases are among heated debate, especially for cancer. To investigate the possible role of mtDNA mutations in lung cancer, we sequenced complete mtDNA of lung cancer tissues, corresponding normal (i.e., non-cancerous) lung tissues, and peripheral blood samples from 55 lung cancer patients and examined the relationship between mtDNA mutations or polymorphisms and clinical parameters. We identified 56 mutations in 33 (60{\%}) of the 55 patients, including 48 point mutations, four single-nucleotide insertions, and four single-nucleotide deletions. Nineteen of these mutations resulted in amino acid substitution. These missense mtDNA mutations were distributed in 9 of 13 mitochondrial DNA coding genes. Three hundred eighty eight polymorphisms were identified among the 55 patients. Seventy-three polymorphisms resulted in amino acid substitution. There was no association of incidence of specific mtDNA mutation or polymorphism with patients' gender, age at diagnosis, smoking history, tumor type or tumor stage (P > 0.05). This study revealed a variety of mtDNA mutations and mtDNA polymorphisms in human lung cancer, some of which might be involved in human lung carcinogenesis.",
keywords = "Lung cancer, Mitochondrial DNA, Mutation",
author = "Xiongjie Jin and Jianjun Zhang and Yanning Gao and Keyue Ding and Naishu Wang and David Zhou and Jin Jen and Shujun Cheng",
year = "2007",
month = "9",
day = "1",
doi = "10.1016/j.mito.2007.06.003",
language = "English (US)",
volume = "7",
pages = "347--353",
journal = "Mitochondrion",
issn = "1567-7249",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer

AU - Jin, Xiongjie

AU - Zhang, Jianjun

AU - Gao, Yanning

AU - Ding, Keyue

AU - Wang, Naishu

AU - Zhou, David

AU - Jen, Jin

AU - Cheng, Shujun

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Mitochondrial DNA (mtDNA) is known for its high frequencies of polymorphisms and mutations, some of which are related to various diseases, including cancers. However, roles of mutations and polymorphisms in some diseases are among heated debate, especially for cancer. To investigate the possible role of mtDNA mutations in lung cancer, we sequenced complete mtDNA of lung cancer tissues, corresponding normal (i.e., non-cancerous) lung tissues, and peripheral blood samples from 55 lung cancer patients and examined the relationship between mtDNA mutations or polymorphisms and clinical parameters. We identified 56 mutations in 33 (60%) of the 55 patients, including 48 point mutations, four single-nucleotide insertions, and four single-nucleotide deletions. Nineteen of these mutations resulted in amino acid substitution. These missense mtDNA mutations were distributed in 9 of 13 mitochondrial DNA coding genes. Three hundred eighty eight polymorphisms were identified among the 55 patients. Seventy-three polymorphisms resulted in amino acid substitution. There was no association of incidence of specific mtDNA mutation or polymorphism with patients' gender, age at diagnosis, smoking history, tumor type or tumor stage (P > 0.05). This study revealed a variety of mtDNA mutations and mtDNA polymorphisms in human lung cancer, some of which might be involved in human lung carcinogenesis.

AB - Mitochondrial DNA (mtDNA) is known for its high frequencies of polymorphisms and mutations, some of which are related to various diseases, including cancers. However, roles of mutations and polymorphisms in some diseases are among heated debate, especially for cancer. To investigate the possible role of mtDNA mutations in lung cancer, we sequenced complete mtDNA of lung cancer tissues, corresponding normal (i.e., non-cancerous) lung tissues, and peripheral blood samples from 55 lung cancer patients and examined the relationship between mtDNA mutations or polymorphisms and clinical parameters. We identified 56 mutations in 33 (60%) of the 55 patients, including 48 point mutations, four single-nucleotide insertions, and four single-nucleotide deletions. Nineteen of these mutations resulted in amino acid substitution. These missense mtDNA mutations were distributed in 9 of 13 mitochondrial DNA coding genes. Three hundred eighty eight polymorphisms were identified among the 55 patients. Seventy-three polymorphisms resulted in amino acid substitution. There was no association of incidence of specific mtDNA mutation or polymorphism with patients' gender, age at diagnosis, smoking history, tumor type or tumor stage (P > 0.05). This study revealed a variety of mtDNA mutations and mtDNA polymorphisms in human lung cancer, some of which might be involved in human lung carcinogenesis.

KW - Lung cancer

KW - Mitochondrial DNA

KW - Mutation

UR - http://www.scopus.com/inward/record.url?scp=34548127859&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548127859&partnerID=8YFLogxK

U2 - 10.1016/j.mito.2007.06.003

DO - 10.1016/j.mito.2007.06.003

M3 - Article

C2 - 17707697

AN - SCOPUS:34548127859

VL - 7

SP - 347

EP - 353

JO - Mitochondrion

JF - Mitochondrion

SN - 1567-7249

IS - 5

ER -