Relationship between the Induction of RAGE Cell-Surface Antigen and the Expression of Amyloid Binding Sites

Shyamala Mruthinti, William D Hill, Satyanarayana Swamy-Mruthinti, Jerry J. Buccafusco

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purified human brain neurofilament protein was subjected to glycating conditions to produce an advanced glycation end product (HNF-AGE). Two mice were immunized with this HNF-AGE. Unexpectedly, the animals generated IgGs against a peptide immunogenic fragment of the receptor for advanced glycation end products (RAGE) and against human amyloid β peptide (Aβ). In leukocyte populations, 30-52% of lymphocytes were positive for cell-surface RAGE, and 20-25% were positive for the Aβ peptide. A monoclonal antibody (MAb) directed against the sequence of human RAGE was reactive against a 35-kDa protein band that was highly expressed in blood cells, plasma proteins, lung, liver, spleen, and brain derived from the immunized mice. A MAb directed against Aβ proteins also identified the same 35-kDa band. Thus, the time-dependent formation of AGEs might play a role within the context of the immune system in the expression of binding sites for amyloid peptides, possibly resulting in enhancing cellular toxicity.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalJournal of Molecular Neuroscience
Volume20
Issue number3
DOIs
StatePublished - Dec 1 2003

Fingerprint

Surface Antigens
Amyloid
Binding Sites
Monoclonal Antibodies
Neurofilament Proteins
Peptides
Advanced Glycosylation End Products
Peptide Fragments
Brain
Cell Surface Receptors
Blood Proteins
Immune System
Blood Cells
Proteins
Leukocytes
Spleen
Lymphocytes
Lung
Liver
Population

Keywords

  • Advanced glycation end products
  • Amyloid
  • Immune response
  • Leukocytes
  • Neurofilament protein

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Relationship between the Induction of RAGE Cell-Surface Antigen and the Expression of Amyloid Binding Sites. / Mruthinti, Shyamala; Hill, William D; Swamy-Mruthinti, Satyanarayana; Buccafusco, Jerry J.

In: Journal of Molecular Neuroscience, Vol. 20, No. 3, 01.12.2003, p. 223-232.

Research output: Contribution to journalArticle

Mruthinti, Shyamala ; Hill, William D ; Swamy-Mruthinti, Satyanarayana ; Buccafusco, Jerry J. / Relationship between the Induction of RAGE Cell-Surface Antigen and the Expression of Amyloid Binding Sites. In: Journal of Molecular Neuroscience. 2003 ; Vol. 20, No. 3. pp. 223-232.
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