Relative activities of acetyl CoA carboxylase and fatty acid synthetase in chick liver: effects of dietary fat

G. I. Liou, W. E. Donaldson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The specific activities of acetyl CoA carboxylase and fatty acid synthetase were measured in the cytosol fraction of livers from chicks fed various levels of corn oil, cottonseed oil, corn oil free fatty acids, or crude (79%) oleic acid. Activities of both enzymes were depressed by the addition of fat to a fat free basal diet. The ratios of synthetase to carboxylase activity were greater than unity when up to 4% fat was fed, but less than unity when 8% or higher levels of fat were fed. The depressions of the activities of these enzymes appeared to be unrelated to the dietary level of linoleate. In in vitro experiments, 2 μM concentrations of palmityl CoA or oleoyl CoA depressed acetyl CoA carboxylase activity. Concentrations of 20 μM of these acyl CoA esters did not affect the activity of fatty acid synthetase.

Original languageEnglish (US)
Pages (from-to)1029-1033
Number of pages5
JournalCanadian Journal of Biochemistry
Volume51
Issue number7
DOIs
StatePublished - Jan 1 1973

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Acetyl-CoA Carboxylase
Fatty Acid Synthases
Dietary Fats
Corn Oil
Fats
Liver
Palmitoyl Coenzyme A
Cottonseed Oil
Acyl Coenzyme A
Fat-Restricted Diet
Linoleic Acid
Enzymes
Oleic Acid
Ligases
Nonesterified Fatty Acids
Cytosol
Esters

ASJC Scopus subject areas

  • Medicine(all)

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Relative activities of acetyl CoA carboxylase and fatty acid synthetase in chick liver : effects of dietary fat. / Liou, G. I.; Donaldson, W. E.

In: Canadian Journal of Biochemistry, Vol. 51, No. 7, 01.01.1973, p. 1029-1033.

Research output: Contribution to journalArticle

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