Remedial effect of lupeol and its ester derivative on hypercholesterolemia-induced oxidative and inflammatory stresses

Sudhahar Varadarajan, Sekar Ashok Kumar, Yenjerla Mythili, Palaninathan Varalakshmi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the hypercholesterolemic condition, the net result of combined lipemic-oxidative and inflammatory aberrations is manifested as an increase in adhesion molecule expression, increased vascular permeability, and the progress of atherogenesis. The aim of the present work is to evaluate the role of lupeol and its ester derivative on oxidative and inflammatory abnormalities in hypercholesterolemic condition. Hypercholesterolemia was induced in male albino rats of Wistar strain by feeding them a high-cholesterol diet (HCD) containing normal rat chow supplemented with 4% cholesterol and 1% cholic acid for 30 days. The pentacyclic triterpene, lupeol, and the ester derivative of lupeol, lupeol linoleate, were supplemented (50 mg/kg body weight per day, PO) during the last 15 days. Oxidative stress in HCD-fed animals was characterized by a significant increase in reactive oxygen species with concomitant decrease in the thiol levels in heart. Cardiac nuclear factor-κB nuclear translocation was confirmed by immunohistochemisty in HCD-fed rats. The tumor necrosis factor-α levels showed an increase in hypercholesterolemic condition, whereas a departure toward control values and decreased nuclear translocation of nuclear factor-κB and oxidative stress were notable in supplementation with triterpenes. The nitric oxide levels and mRNA expression for inducible nitric oxide synthase was also significantly increased in HCD-fed animals. Treatment with lupeol and lupeol linoleate reversed the nitrosative stress to near normalcy. These observations highlight the beneficial effects of the triterpene, lupeol, and linoleate ester in ameliorating the oxidative and inflammatory abnormalities in the hypercholesterolemic conditions.

Original languageEnglish (US)
Pages (from-to)778-787
Number of pages10
JournalNutrition Research
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2007

Fingerprint

Hypercholesterolemia
Esters
Oxidative Stress
Cholesterol
Diet
Triterpenes
Pentacyclic Triterpenes
Cholic Acid
Capillary Permeability
Nitric Oxide Synthase Type II
Sulfhydryl Compounds
Wistar Rats
Reactive Oxygen Species
Atherosclerosis
Nitric Oxide
Tumor Necrosis Factor-alpha
Body Weight
lupeol
Messenger RNA
lupeol linoleate

Keywords

  • High-cholesterol diet
  • Nitrosative stress
  • Oxidative stress
  • Pentacyclic triterpene
  • Rats

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Remedial effect of lupeol and its ester derivative on hypercholesterolemia-induced oxidative and inflammatory stresses. / Varadarajan, Sudhahar; Kumar, Sekar Ashok; Mythili, Yenjerla; Varalakshmi, Palaninathan.

In: Nutrition Research, Vol. 27, No. 12, 01.12.2007, p. 778-787.

Research output: Contribution to journalArticle

Varadarajan, Sudhahar ; Kumar, Sekar Ashok ; Mythili, Yenjerla ; Varalakshmi, Palaninathan. / Remedial effect of lupeol and its ester derivative on hypercholesterolemia-induced oxidative and inflammatory stresses. In: Nutrition Research. 2007 ; Vol. 27, No. 12. pp. 778-787.
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AB - In the hypercholesterolemic condition, the net result of combined lipemic-oxidative and inflammatory aberrations is manifested as an increase in adhesion molecule expression, increased vascular permeability, and the progress of atherogenesis. The aim of the present work is to evaluate the role of lupeol and its ester derivative on oxidative and inflammatory abnormalities in hypercholesterolemic condition. Hypercholesterolemia was induced in male albino rats of Wistar strain by feeding them a high-cholesterol diet (HCD) containing normal rat chow supplemented with 4% cholesterol and 1% cholic acid for 30 days. The pentacyclic triterpene, lupeol, and the ester derivative of lupeol, lupeol linoleate, were supplemented (50 mg/kg body weight per day, PO) during the last 15 days. Oxidative stress in HCD-fed animals was characterized by a significant increase in reactive oxygen species with concomitant decrease in the thiol levels in heart. Cardiac nuclear factor-κB nuclear translocation was confirmed by immunohistochemisty in HCD-fed rats. The tumor necrosis factor-α levels showed an increase in hypercholesterolemic condition, whereas a departure toward control values and decreased nuclear translocation of nuclear factor-κB and oxidative stress were notable in supplementation with triterpenes. The nitric oxide levels and mRNA expression for inducible nitric oxide synthase was also significantly increased in HCD-fed animals. Treatment with lupeol and lupeol linoleate reversed the nitrosative stress to near normalcy. These observations highlight the beneficial effects of the triterpene, lupeol, and linoleate ester in ameliorating the oxidative and inflammatory abnormalities in the hypercholesterolemic conditions.

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