Renal 20-hydroxyeicosatetraenoic acid synthesis during pregnancy

Mong-Heng Wang, Barbara A. Zand, Alberto Nasjletti, Michal Laniado-Schwartzman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We examined whether renal 20-hydroxyeicosatetraenoic acid (20-HETE) synthesis is altered during gestation. Renal microsomal arachidonic acid ω-hydroxylase activity increased by 50 and 48% in rats on days 12 and 19 of gestation, respectively. Renal microvessel 20-HETE synthesis increased by 50 and 82% in rats on days 6 and 12 of gestation, respectively, and returned to control levels at day 19 of gestation. In contrast, 20-HETE synthesis in isolated medullary thick ascending limb was unchanged from control levels on days 6 and 12 of gestation, but it increased twofold on day 19 of gestation. This increase on day 19 of gestation was associated with a twofold increase in urinary 20-HETE excretion, and it coincided with a 23-mmHg fall in blood pressure. Moreover, change in the rate of 20-HETE synthesis in microvessels was consistent with the level of expression of cytochrome P450 (CYP)4A proteins. Administration of the CYP4A inhibitor 1-aminobenzotriazole (ABT) for 2 days on day 12 of pregnancy or for 5 days starting on day 15 of pregnancy caused a transient but significant reduction in systolic blood pressure. ABT treatment also decreased urinary sodium, urinary 20-HETE, and renal and microvessel 20-HETE synthesis. This study, to our knowledge, is the first to demonstrate that 20-HETE synthesis in the kidney is altered in time- and site-specific manners during pregnancy. The localized pattern of changes suggests that there are distinct regulatory mechanisms for 20-HETE synthesis in the kidney during pregnancy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number2 51-2
StatePublished - Jul 2 2002
Externally publishedYes

Fingerprint

Kidney
Pregnancy
Microvessels
Blood Pressure
20-hydroxy-5,8,11,14-eicosatetraenoic acid
Cytochrome P-450 CYP4A
Mixed Function Oxygenases
Arachidonic Acid
Cytochrome P-450 Enzyme System
Extremities
Sodium
Proteins

Keywords

  • 1-aminobenzotriazole
  • Medullary thick ascending limb
  • Renal microvessels

ASJC Scopus subject areas

  • Physiology

Cite this

Renal 20-hydroxyeicosatetraenoic acid synthesis during pregnancy. / Wang, Mong-Heng; Zand, Barbara A.; Nasjletti, Alberto; Laniado-Schwartzman, Michal.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 282, No. 2 51-2, 02.07.2002.

Research output: Contribution to journalArticle

Wang, Mong-Heng ; Zand, Barbara A. ; Nasjletti, Alberto ; Laniado-Schwartzman, Michal. / Renal 20-hydroxyeicosatetraenoic acid synthesis during pregnancy. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2002 ; Vol. 282, No. 2 51-2.
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