Renal delivery of anti-microrna oligonucleotides in rats

Kristie S. Usa, Yong Liu, Terry Kurth, Alison J. Kriegel, David L. Mattson, Allen W. Cowley, Mingyu Liang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs are endogenous small, non-protein-coding RNA molecules that play an important role in the regulation of a wide variety of cellular functions and disease processes. A novel role for microRNAs in the development of hypertension and hypertensive tissue injury is emerging in recent studies. Development of hypertension involves multiple organ systems and cannot be modeled in vitro. Therefore, the ability to experimentally alter genes, gene products, or biological pathways, including microRNAs, in an organ-specific manner in intact animal models is particularly valuable to hypertension research. The kidney plays a central role in the long-term regulation of arterial blood pressure. In this chapter, we describe a detailed protocol for using a renal interstitial injection method to deliver anti-miR oligonucleotides to knock down microRNA specifically in the kidney in conscious rats.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages409-419
Number of pages11
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1527
ISSN (Print)1064-3745

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Keywords

  • Gene expression
  • Kidney
  • MicroRNA
  • Oligonucleotides
  • Rat

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Usa, K. S., Liu, Y., Kurth, T., Kriegel, A. J., Mattson, D. L., Cowley, A. W., & Liang, M. (2017). Renal delivery of anti-microrna oligonucleotides in rats. In Methods in Molecular Biology (pp. 409-419). (Methods in Molecular Biology; Vol. 1527). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6625-7_31