Renal medullary tissue oxygenation is dependent on both cortical and medullary blood flow

Paul M O'Connor, Michelle M. Kett, Warwick P. Anderson, Roger G. Evans

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The aim of the current study was to determine whether renal medullary oxygenation is independent of the level of cortical blood flow by testing responses to stimuli that selectively reduce blood flow in either the cortex or medulla. In anesthetized rabbits, renal arterial infusion of [Phe 2,Ile3,Orn8]-vasopressin selectively reduced medullary perfusion and PO2 (-54 ± 24 and -50 ± 10%, respectively) but did not significantly affect cortical perfusion or tissue oxygenation. In contrast, stimulation of the renal nerves resulted in renal cortical ischemia with reductions in total renal blood flow (-76 ± 3% at 4 Hz), cortical perfusion (-57 ± 17%), and cortical PO2 (-44 ± 12%). Medullary tissue PO2 was reduced by -70 ± 5% at 4 Hz, despite medullary perfusion being unaffected and distal tubular sodium reabsorption being reduced (by -83.3 ± 1.2% from baseline). In anesthetized rats, in which renal perfusion pressure was maintained with an aortic constrictor, intravenous infusion of ANG II (0.5-5 μg·kg -1·min-1) dose dependently reduced cortical perfusion (up to -65 ± 3%; P < 0.001) and cortical PO2 (up to -57 ± 4%; P < 0.05). However, medullary perfusion was only significantly reduced at the highest dose (5 μg·kg -1·min-1; by 29 ± 6%). Medullary perfusion was not reduced by 1 μg·kg-1·min-1 ANG II, but medullary PO2 was significantly reduced (-12 ± 4%). Thus, although cortical and medullary blood flow may be independently regulated, medullary oxygenation may be compromised during moderate to severe cortical ischemia even when medullary blood flow is maintained.

Original languageEnglish (US)
Pages (from-to)F688-F694
JournalAmerican Journal of Physiology - Renal Physiology
Volume290
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Fingerprint

Perfusion
Kidney
Ischemia
Renal Circulation
Vasopressins
Intravenous Infusions
Sodium
Rabbits
Pressure

Keywords

  • Acute renal failure
  • Acute tubular necrosis
  • Fluorescence oximetry
  • Hypoxia
  • Ischemia
  • Laser-Doppler flowmetry
  • Oxygen
  • Rabbits
  • Rats
  • Renal circulation

ASJC Scopus subject areas

  • Physiology

Cite this

Renal medullary tissue oxygenation is dependent on both cortical and medullary blood flow. / O'Connor, Paul M; Kett, Michelle M.; Anderson, Warwick P.; Evans, Roger G.

In: American Journal of Physiology - Renal Physiology, Vol. 290, No. 3, 01.03.2006, p. F688-F694.

Research output: Contribution to journalArticle

O'Connor, Paul M ; Kett, Michelle M. ; Anderson, Warwick P. ; Evans, Roger G. / Renal medullary tissue oxygenation is dependent on both cortical and medullary blood flow. In: American Journal of Physiology - Renal Physiology. 2006 ; Vol. 290, No. 3. pp. F688-F694.
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