Renal nerves and leukocyte infiltration in the kidney during salt-sensitive hypertension

Ammar J. Alsheikh, Hayley Lund, John Henry Dasinger, Justine M. Abais-Battad, Daniel J. Fehrenbach, David L. Mattson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Based on previous studies suggesting a role of renal nerves in renal inflammation, the present studies were performed to test the hypothesis that renal nerves mediate renal damage in Dahl salt-sensitive (SS) hypertension by increasing renal leukocyte infiltration. Experiments were performed in Dahl SS rats with bilateral renal denervation (RDN) and bilateral sham operation (n ± 10 or 11 per group) and with unilateral RDN and contralateral sham operation (n ± 10). After denervation, rats were switched from a low-salt 0.4% NaCl (LS) diet to a high-salt 4% NaCl (HS) diet and maintained on HS diet for 21 days. Bilateral RDN reduced the magnitude of hypertension assessed by radiotelemetry in Dahl SS rats compared with sham-operated rats (mean arterial pressure 140.9 > 4.8 mmHg and 159.7 > 3.5 mmHg, respectively) and reduced proteinuria at day 21 of HS diet. However, assessment of renal leukocyte infiltration demonstrated no significant effect of bilateral RDN on the number of infiltrating leukocytes (RDN 3.6 > 0.5 < 106 vs. sham operated 4.3 > 0.3 < 106 CD45< cells) or any of the subsets examined by flow cytometry. The unilateral RDN experiment showed no effect of RDN on the renal infiltration of leukocytes (RDN 6.5 > 0.9 < 106 vs. sham operated 6.1 > 1.1 < 106 CD45< cells/ kidney) or renal damage in RDN vs. sham-operated kidney after 21 days of HS diet. This work investigated the relationship between renal nerves and renal inflammation during Dahl SS hypertension. Contrary to our hypothesis, the results of this work suggest that immune cell infiltration in the kidney of Dahl SS rats is not mediated by the renal nerves.

Original languageEnglish (US)
Pages (from-to)R182-R189
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Issue number1
StatePublished - 2019
Externally publishedYes


  • Dahl salt-sensitive rat
  • Hypertension
  • Leukocytes
  • Renal damage
  • Renal nerves

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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