Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats

Jennifer C. Sullivan, Jennifer L. Pardieck, Kelly A. Hyndman, Jennifer S. Pollock

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The goal of this study was to examine the status of the renal nitric oxide (NO) system by determining NO synthase (NOS) isoform activity and expression within the three regions of the kidney in 14-wk-old male and female spontaneously hypertensive rats (SHR). NOS activity, and NOS1 and NOS3 protein expressions and localization were comparable in the renal cortex and outer medulla of male and female SHR. In contrast, male SHR had significantly less NOS1 and NOS3 enzymatic activity (0 ± 5 and 53 ± 7 pmol•mg-1 •30 min-1, respectively) compared with female SHR (37 ± 16 and 172 ± 40 pmol•mg-1 •30 min-1, respectively). Lower levels of inner medullary NOS1 activity in male SHR were associated with less NOS1 protein expression [45 ± 7 relative densitometric units (RDU)] and fewer NOS1-positive cells in the renal inner medulla compared with female SHR (79 ± 12 RDU). Phosphorylation of NOS3 is an important determinant of NOS activity. Male SHR had significantly greater phosphorylation of NOS3 on threonine 495 in the renal cortex compared with females (0.25 ± 0.05 vs. 0.15 ± 0.06 RDU). NOS3 phosphorylation was comparable in males and females in the other regions of the kidney. cGMP levels were measured as an indirect index of NO production. cGMP levels were significantly lower in the renal cortex (0.08 ± 0.01 pmol/mg) and inner medulla (0.43 ± 0.02 pmol/mg) of male SHR compared with females (cortex: 0.14 ± 0.02 pmol/mg; inner medulla: 0.56 ± 0.02 pmol/mg). Our data suggest that the effect of the sex of the animal on NOS activity and expression is different in the three regions of the SHR kidney and supports the hypothesis that male SHR have lower NO bioavailability compared with females.

Original languageEnglish (US)
Pages (from-to)R61-R69
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume298
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Inbred SHR Rats
Nitric Oxide Synthase
Kidney
Nitric Oxide Synthase Type I
Nitric Oxide
Phosphorylation
Threonine
Biological Availability
Protein Isoforms

Keywords

  • Cgmp
  • Gender
  • Kidney
  • Nitric oxide synthase
  • Sex

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats. / Sullivan, Jennifer C.; Pardieck, Jennifer L.; Hyndman, Kelly A.; Pollock, Jennifer S.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 298, No. 1, 01.01.2010, p. R61-R69.

Research output: Contribution to journalArticle

@article{8424fb1b910f4f2a8a5c690a8e7d2e25,
title = "Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats",
abstract = "The goal of this study was to examine the status of the renal nitric oxide (NO) system by determining NO synthase (NOS) isoform activity and expression within the three regions of the kidney in 14-wk-old male and female spontaneously hypertensive rats (SHR). NOS activity, and NOS1 and NOS3 protein expressions and localization were comparable in the renal cortex and outer medulla of male and female SHR. In contrast, male SHR had significantly less NOS1 and NOS3 enzymatic activity (0 ± 5 and 53 ± 7 pmol•mg-1 •30 min-1, respectively) compared with female SHR (37 ± 16 and 172 ± 40 pmol•mg-1 •30 min-1, respectively). Lower levels of inner medullary NOS1 activity in male SHR were associated with less NOS1 protein expression [45 ± 7 relative densitometric units (RDU)] and fewer NOS1-positive cells in the renal inner medulla compared with female SHR (79 ± 12 RDU). Phosphorylation of NOS3 is an important determinant of NOS activity. Male SHR had significantly greater phosphorylation of NOS3 on threonine 495 in the renal cortex compared with females (0.25 ± 0.05 vs. 0.15 ± 0.06 RDU). NOS3 phosphorylation was comparable in males and females in the other regions of the kidney. cGMP levels were measured as an indirect index of NO production. cGMP levels were significantly lower in the renal cortex (0.08 ± 0.01 pmol/mg) and inner medulla (0.43 ± 0.02 pmol/mg) of male SHR compared with females (cortex: 0.14 ± 0.02 pmol/mg; inner medulla: 0.56 ± 0.02 pmol/mg). Our data suggest that the effect of the sex of the animal on NOS activity and expression is different in the three regions of the SHR kidney and supports the hypothesis that male SHR have lower NO bioavailability compared with females.",
keywords = "Cgmp, Gender, Kidney, Nitric oxide synthase, Sex",
author = "Sullivan, {Jennifer C.} and Pardieck, {Jennifer L.} and Hyndman, {Kelly A.} and Pollock, {Jennifer S.}",
year = "2010",
month = "1",
day = "1",
doi = "10.1152/ajpregu.00526.2009",
language = "English (US)",
volume = "298",
pages = "R61--R69",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats

AU - Sullivan, Jennifer C.

AU - Pardieck, Jennifer L.

AU - Hyndman, Kelly A.

AU - Pollock, Jennifer S.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The goal of this study was to examine the status of the renal nitric oxide (NO) system by determining NO synthase (NOS) isoform activity and expression within the three regions of the kidney in 14-wk-old male and female spontaneously hypertensive rats (SHR). NOS activity, and NOS1 and NOS3 protein expressions and localization were comparable in the renal cortex and outer medulla of male and female SHR. In contrast, male SHR had significantly less NOS1 and NOS3 enzymatic activity (0 ± 5 and 53 ± 7 pmol•mg-1 •30 min-1, respectively) compared with female SHR (37 ± 16 and 172 ± 40 pmol•mg-1 •30 min-1, respectively). Lower levels of inner medullary NOS1 activity in male SHR were associated with less NOS1 protein expression [45 ± 7 relative densitometric units (RDU)] and fewer NOS1-positive cells in the renal inner medulla compared with female SHR (79 ± 12 RDU). Phosphorylation of NOS3 is an important determinant of NOS activity. Male SHR had significantly greater phosphorylation of NOS3 on threonine 495 in the renal cortex compared with females (0.25 ± 0.05 vs. 0.15 ± 0.06 RDU). NOS3 phosphorylation was comparable in males and females in the other regions of the kidney. cGMP levels were measured as an indirect index of NO production. cGMP levels were significantly lower in the renal cortex (0.08 ± 0.01 pmol/mg) and inner medulla (0.43 ± 0.02 pmol/mg) of male SHR compared with females (cortex: 0.14 ± 0.02 pmol/mg; inner medulla: 0.56 ± 0.02 pmol/mg). Our data suggest that the effect of the sex of the animal on NOS activity and expression is different in the three regions of the SHR kidney and supports the hypothesis that male SHR have lower NO bioavailability compared with females.

AB - The goal of this study was to examine the status of the renal nitric oxide (NO) system by determining NO synthase (NOS) isoform activity and expression within the three regions of the kidney in 14-wk-old male and female spontaneously hypertensive rats (SHR). NOS activity, and NOS1 and NOS3 protein expressions and localization were comparable in the renal cortex and outer medulla of male and female SHR. In contrast, male SHR had significantly less NOS1 and NOS3 enzymatic activity (0 ± 5 and 53 ± 7 pmol•mg-1 •30 min-1, respectively) compared with female SHR (37 ± 16 and 172 ± 40 pmol•mg-1 •30 min-1, respectively). Lower levels of inner medullary NOS1 activity in male SHR were associated with less NOS1 protein expression [45 ± 7 relative densitometric units (RDU)] and fewer NOS1-positive cells in the renal inner medulla compared with female SHR (79 ± 12 RDU). Phosphorylation of NOS3 is an important determinant of NOS activity. Male SHR had significantly greater phosphorylation of NOS3 on threonine 495 in the renal cortex compared with females (0.25 ± 0.05 vs. 0.15 ± 0.06 RDU). NOS3 phosphorylation was comparable in males and females in the other regions of the kidney. cGMP levels were measured as an indirect index of NO production. cGMP levels were significantly lower in the renal cortex (0.08 ± 0.01 pmol/mg) and inner medulla (0.43 ± 0.02 pmol/mg) of male SHR compared with females (cortex: 0.14 ± 0.02 pmol/mg; inner medulla: 0.56 ± 0.02 pmol/mg). Our data suggest that the effect of the sex of the animal on NOS activity and expression is different in the three regions of the SHR kidney and supports the hypothesis that male SHR have lower NO bioavailability compared with females.

KW - Cgmp

KW - Gender

KW - Kidney

KW - Nitric oxide synthase

KW - Sex

UR - http://www.scopus.com/inward/record.url?scp=73549096620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73549096620&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00526.2009

DO - 10.1152/ajpregu.00526.2009

M3 - Article

C2 - 19889864

AN - SCOPUS:73549096620

VL - 298

SP - R61-R69

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 1

ER -