Renal Proteome in Mice with Different Susceptibilities to Fluorosis

Juliane Guimarães Carvalho, Aline de Lima Leite, Camila Peres-Buzalaf, Fernanda Salvato, Carlos Alberto Labate, Eric T. Everett, Gary M. Whitford, Marília Afonso Rabelo Buzalaf

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis due to their genetic backgrounds. They also differ with respect to several features of fluoride (F) metabolism and metabolic handling of water. This study was done to determine whether differences in F metabolism could be explained by diversities in the profile of protein expression in kidneys. Weanling, male A/J mice (susceptible to dental fluorosis, n = 18) and 129P3/J mice (resistant, n = 18) were housed in pairs and assigned to three groups given low-F food and drinking water containing 0, 10 or 50 ppm [F] for 7 weeks. Renal proteome profiles were examined using 2D-PAGE and LC-MS/MS. Quantitative intensity analysis detected between A/J and 129P3/J strains 122, 126 and 134 spots differentially expressed in the groups receiving 0, 10 and 50 ppmF, respectively. From these, 25, 30 and 32, respectively, were successfully identified. Most of the proteins were related to metabolic and cellular processes, followed by response to stimuli, development and regulation of cellular processes. In F-treated groups, PDZK-1, a protein involved in the regulation of renal tubular reabsorption capacity was down-modulated in the kidney of 129P3/J mice. A/J and 129P3/J mice exhibited 11 and 3 exclusive proteins, respectively, regardless of F exposure. In conclusion, proteomic analysis was able to identify proteins potentially involved in metabolic handling of F and water that are differentially expressed or even not expressed in the strains evaluated. This can contribute to understanding the molecular mechanisms underlying genetic susceptibility to dental fluorosis, by indicating key-proteins that should be better addressed in future studies.

Original languageEnglish (US)
Article numbere53261
JournalPloS one
Volume8
Issue number1
DOIs
StatePublished - Jan 4 2013

Fingerprint

fluorosis
Proteome
proteome
kidneys
Dental Fluorosis
Kidney
mice
teeth
Proteins
proteins
Metabolism
metabolism
fluorides
weanlings
Water
proteomics
genetic background
Electrophoresis, Gel, Two-Dimensional
drinking water
Genetic Predisposition to Disease

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Carvalho, J. G., Leite, A. D. L., Peres-Buzalaf, C., Salvato, F., Labate, C. A., Everett, E. T., ... Buzalaf, M. A. R. (2013). Renal Proteome in Mice with Different Susceptibilities to Fluorosis. PloS one, 8(1), [e53261]. https://doi.org/10.1371/journal.pone.0053261

Renal Proteome in Mice with Different Susceptibilities to Fluorosis. / Carvalho, Juliane Guimarães; Leite, Aline de Lima; Peres-Buzalaf, Camila; Salvato, Fernanda; Labate, Carlos Alberto; Everett, Eric T.; Whitford, Gary M.; Buzalaf, Marília Afonso Rabelo.

In: PloS one, Vol. 8, No. 1, e53261, 04.01.2013.

Research output: Contribution to journalArticle

Carvalho, JG, Leite, ADL, Peres-Buzalaf, C, Salvato, F, Labate, CA, Everett, ET, Whitford, GM & Buzalaf, MAR 2013, 'Renal Proteome in Mice with Different Susceptibilities to Fluorosis', PloS one, vol. 8, no. 1, e53261. https://doi.org/10.1371/journal.pone.0053261
Carvalho JG, Leite ADL, Peres-Buzalaf C, Salvato F, Labate CA, Everett ET et al. Renal Proteome in Mice with Different Susceptibilities to Fluorosis. PloS one. 2013 Jan 4;8(1). e53261. https://doi.org/10.1371/journal.pone.0053261
Carvalho, Juliane Guimarães ; Leite, Aline de Lima ; Peres-Buzalaf, Camila ; Salvato, Fernanda ; Labate, Carlos Alberto ; Everett, Eric T. ; Whitford, Gary M. ; Buzalaf, Marília Afonso Rabelo. / Renal Proteome in Mice with Different Susceptibilities to Fluorosis. In: PloS one. 2013 ; Vol. 8, No. 1.
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