Abstract
Objective: To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum. Design: Prospective laboratory study. Setting: Academic medical center. Patient(s): Four men and three pregnant women in the third trimester. Intervention(s): Pooled serum from men and pregnant females were frozen at either -20°C or -70°C. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle. Main Outcome Measure(s): Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17α-hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature. Result(s): Only SHBG in male serum at -20°C and P in pregnant serum at -70°C showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3% and 1.1% per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature. Conclusion(s): There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum.
Original language | English (US) |
---|---|
Pages (from-to) | 823-825 |
Number of pages | 3 |
Journal | Fertility and sterility |
Volume | 76 |
Issue number | 4 |
DOIs | |
State | Published - 2001 |
Keywords
- 17α-hydroxyprogesterone
- Androstenedione
- Cryostorage
- FSH
- Glucose
- Hormones
- Insulin
- LH
- Measurement
- P
- Prolactin
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology