Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones

Rosario Reyna, Kyle D. Traynor, Gene Hines, Larry R. Boots, Ricardo Azziz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum. Design: Prospective laboratory study. Setting: Academic medical center. Patient(s): Four men and three pregnant women in the third trimester. Intervention(s): Pooled serum from men and pregnant females were frozen at either -20°C or -70°C. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle. Main Outcome Measure(s): Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17α-hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature. Result(s): Only SHBG in male serum at -20°C and P in pregnant serum at -70°C showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3% and 1.1% per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature. Conclusion(s): There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum.

Original languageEnglish (US)
Pages (from-to)823-825
Number of pages3
JournalFertility and sterility
Volume76
Issue number4
DOIs
StatePublished - Oct 16 2001

Fingerprint

Sex Hormone-Binding Globulin
Freezing
Hormones
Serum
Temperature
17-alpha-Hydroxyprogesterone
Androstenedione
Third Pregnancy Trimester
Pregnant Women
Glycoproteins
Steroids
Outcome Assessment (Health Care)
Prospective Studies
Insulin

Keywords

  • 17α-hydroxyprogesterone
  • Androstenedione
  • Cryostorage
  • FSH
  • Glucose
  • Hormones
  • Insulin
  • LH
  • Measurement
  • P
  • Prolactin

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones. / Reyna, Rosario; Traynor, Kyle D.; Hines, Gene; Boots, Larry R.; Azziz, Ricardo.

In: Fertility and sterility, Vol. 76, No. 4, 16.10.2001, p. 823-825.

Research output: Contribution to journalArticle

Reyna, Rosario ; Traynor, Kyle D. ; Hines, Gene ; Boots, Larry R. ; Azziz, Ricardo. / Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones. In: Fertility and sterility. 2001 ; Vol. 76, No. 4. pp. 823-825.
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abstract = "Objective: To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum. Design: Prospective laboratory study. Setting: Academic medical center. Patient(s): Four men and three pregnant women in the third trimester. Intervention(s): Pooled serum from men and pregnant females were frozen at either -20°C or -70°C. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle. Main Outcome Measure(s): Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17α-hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature. Result(s): Only SHBG in male serum at -20°C and P in pregnant serum at -70°C showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3{\%} and 1.1{\%} per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature. Conclusion(s): There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum.",
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