Repression of versican expression by microRNA-143

Xiaobo Wang, Guoqing Hu, Jiliang Zhou

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Smooth muscle cells (SMCs) retain remarkable plasticity to undergo phenotypic modulation in which the expression of smooth muscle markers is markedly attenuated while conversely expression of extracellular matrix (ECM) is dramatically up-regulated. Myocardin is perhaps the most potent transcription factor for stimulating expression of smooth muscle-specific genes; little is known, however, about whether myocardin can orchestrate ECM expression to act in concert with smooth muscle differentiation program. In this study, we demonstrated myocardin coordinate smooth muscle differentiation by inducing transcription of microRNA-143 (miR-143), which attenuates ECM versican protein expression. Previous studies have shown that versican is a chondroitin sulfate proteoglycan of the ECM that is produced by synthetic SMCs and promotes SMC migration and proliferation. Our data demonstrated that myocardin significantly represses versican expression in multiple cell lines, and this occurs through the induction of miR-143 by myocardin. By a modified reverse transcribed PCR, we found that miR-143 specifically binds to the 3′-untranslated region of versican mRNA. Reporter assays validated that miR-143 targets versican 3′-untranslated region through an evolutionarily conserved miR-143 binding site. Furthermore, overexpression of miR-143 significantly represses versican expression, whereas conversely, depletion of endogenous miR-143 results in up-regulation of versican expression. In addition, we demonstrated that myocardin represses versican through induction of miR-143. Finally, we found that the regulation of versican by miR-143 is involved in platelet-derived growth factor BB-induced SMC migration. This study provides the first evidence that myocardin, in addition to activating smooth muscle-specific genes, regulates ECM expression through induction of microRNAs during smooth muscle differentiation.

Original languageEnglish (US)
Pages (from-to)23241-23250
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number30
DOIs
StatePublished - Jul 23 2010

Fingerprint

Versicans
MicroRNAs
Muscle
Smooth Muscle
Smooth Muscle Myocytes
Extracellular Matrix
3' Untranslated Regions
Cell Movement
Genes
Cells
Chondroitin Sulfate Proteoglycans
Extracellular Matrix Proteins
Transcription
myocardin
Plasticity
Assays
Transcription Factors
Up-Regulation
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Repression of versican expression by microRNA-143. / Wang, Xiaobo; Hu, Guoqing; Zhou, Jiliang.

In: Journal of Biological Chemistry, Vol. 285, No. 30, 23.07.2010, p. 23241-23250.

Research output: Contribution to journalArticle

Wang, Xiaobo ; Hu, Guoqing ; Zhou, Jiliang. / Repression of versican expression by microRNA-143. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 30. pp. 23241-23250.
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