Requirement for annexin A1 in plasma membrane repair

Anna K. McNeil, Ursula Rescher, Volker Gerke, Paul L McNeil

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Ca2+ entering a cell through a torn or disrupted plasma membrane rapidly triggers a combination of homotypic and exocytotic membrane fusion events. These events serve to erect a reparative membrane patch and then anneal it to the defect site. Annexin A1 is a cytosolic protein that, when activated by micromolar Ca2+, binds to membrane phospholipids, promoting membrane aggregation and fusion. We demonstrate here that an annexin A1 function-blocking antibody, a small peptide competitor, and a dominant-negative annexin A1 mutant protein incapable of Ca2+ binding all inhibit resealing. Moreover, we show that, coincident with a resealing event, annexin A1 becomes concentrated at disruption sites. We propose that Ca2+ entering through a disruption locally induces annexin A1 binding to membranes, initiating emergency fusion events whenever and wherever required.

Original languageEnglish (US)
Pages (from-to)35202-35207
Number of pages6
JournalJournal of Biological Chemistry
Volume281
Issue number46
DOIs
StatePublished - Nov 17 2006

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Annexin A1
Cell membranes
Repair
Cell Membrane
Membranes
Membrane Fusion
Fusion reactions
Blocking Antibodies
Mutant Proteins
Phospholipids
Emergencies
Agglomeration
Defects
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

McNeil, A. K., Rescher, U., Gerke, V., & McNeil, P. L. (2006). Requirement for annexin A1 in plasma membrane repair. Journal of Biological Chemistry, 281(46), 35202-35207. https://doi.org/10.1074/jbc.M606406200

Requirement for annexin A1 in plasma membrane repair. / McNeil, Anna K.; Rescher, Ursula; Gerke, Volker; McNeil, Paul L.

In: Journal of Biological Chemistry, Vol. 281, No. 46, 17.11.2006, p. 35202-35207.

Research output: Contribution to journalArticle

McNeil, AK, Rescher, U, Gerke, V & McNeil, PL 2006, 'Requirement for annexin A1 in plasma membrane repair', Journal of Biological Chemistry, vol. 281, no. 46, pp. 35202-35207. https://doi.org/10.1074/jbc.M606406200
McNeil, Anna K. ; Rescher, Ursula ; Gerke, Volker ; McNeil, Paul L. / Requirement for annexin A1 in plasma membrane repair. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 46. pp. 35202-35207.
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