Requirement of translation but not transcription for the maintenance of long-term depression in the CA1 region of freely moving rats

D. Manahan-Vaughan, A. Kulla, Julietta Uta Frey

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Hippocampal long-term depression (LTD) comprises a persistent reduction in synaptic strength that can be induced in the CA1 region by repeated low-frequency stimulation (LFS). Previous studies have demonstrated that hippocampal long-term potentiation requires de novo protein synthesis. Whether hippocampal LTD is also protein synthesis-dependent is not known. In this study, we investigated if the previous administration of translation inhibitors (anisomycin or emetine) or a transcription inhibitor (actinomycin-D) influenced the profile of LTD in freely moving adult Wistar rats. Seven- to 8-week-old animals underwent chronic implantation of a recording electrode in the CA1 stratum radiatum and a stimulation electrode in the Schaffer collateral/commissural fiber pathway. A cannula was implanted in the ipsilateral cerebral ventricle to enable drug administration. Experiments were commenced 10 d after the implantation procedure. Immediately after application of LFS (1 Hz, 900 pulses) robust LTD was seen that persisted for >8 hr in control animals. Application of anisomycin (240 μg/5 μl) emetine (240 μg/5 μl) before LFS prevented the expression of LTD or ~4.5 hr after LFS. Previous administration of actinomycin D (72 μg/12 μl) had no effect on the expression of LTD. None of the compounds elicited significant effects on basal synaptic transmission when administered in the absence of LFS. These data suggest that LTD in the CA1 region in vivo is protein synthesis-dependent. Furthermore, persistent LTD can be established through the translation of existing mRNA, whereas de novo mRNA transcription does not appear to be necessary.

Original languageEnglish (US)
Pages (from-to)8572-8576
Number of pages5
JournalJournal of Neuroscience
Volume20
Issue number22
StatePublished - Nov 15 2000

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Emetine
Anisomycin
Dactinomycin
Electrodes
Hippocampal CA1 Region
Cerebral Ventricles
Proteins
Long-Term Potentiation
Protein Biosynthesis
Synaptic Transmission
Wistar Rats
Hippocampus
Messenger RNA
Pharmaceutical Preparations
Cannula

Keywords

  • Actinomycin D
  • Anisomycin
  • Hippocampus
  • Long-term depression
  • Protein synthesis
  • Transcription
  • Translation
  • Wistar
  • mRNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Requirement of translation but not transcription for the maintenance of long-term depression in the CA1 region of freely moving rats. / Manahan-Vaughan, D.; Kulla, A.; Frey, Julietta Uta.

In: Journal of Neuroscience, Vol. 20, No. 22, 15.11.2000, p. 8572-8576.

Research output: Contribution to journalArticle

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