Resin adhesion to carious dentin

Masahiro Yoshiyama, Franklin R. Tay, Yasuhiro Torii, Yoshihiro Nishitani, Junichi Doi, Kousuke Itou, Bernard Ciucchi, David H. Pashley

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Purpose: To investigate the microtensile bond strength (μTBS) of a self-etching priming adhesive system to normal, caries-affected and caries-infected dentin, and to observe the ultrastructure of the resin-dentin interface by transmission electron microscopy (TEM). Materials and Methods: Twelve extracted human molar teeth with deep occlusal caries were stained with caries detector solution and ground flat occlusally. The red-stained soft dentin was classified as caries-infected. The surrounding discolored dentin was classified as caries-affected dentin. The surrounding normal dentin served as a control. The entire flat surface was bonded with Clearfil Liner Bond 2V (CV) and covered with resin composite to form a composite crown 5 mm high. One day later the specimens were serially sectioned vertically into multiple slabs 0.8 mm thick. Under microscopic observation, the specimens were divided into normal or caries-infected or caries-affected dentin. These regions were isolated by cutting away the remaining dentin to form hour-glass shapes with the smallest surface area at the test site. After measuring the areas, the specimens were fixed to a microtensile tester and pulled under tension to failure. Additional slabs that were not used for bond strength tests were processed for TEM. Bond strength data were analyzed by Kruskal-Wallis one-way ANOVA and Student-Newman-Keuls multiple comparisons. Results: The μTBS of CV to normal, caries-affected and caries-infected dentin were 45 ± 10 MPa, 30 ± 10 MPa, 10 ± 5 MPa, respectively. TEM images showed that CV formed thin hybrid layers that were less than 1 μm thick in normal dentin, but that were between 6-8 μm thick in caries-affected dentin. Bacteria were only sparsely observed in the dentin tubules of bonded caries-affected dentin. However, in caries-infected dentin, an unusual interface was seen in which carious bacteria within disorganized non-banded collagen fibrils could be seen embedded by the adhesive. The hybrid layer in caries-infected dentin was found to be 30-60 μm thick.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalAmerican journal of dentistry
Volume16
Issue number1
StatePublished - Feb 2003

ASJC Scopus subject areas

  • Dentistry(all)

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    Yoshiyama, M., Tay, F. R., Torii, Y., Nishitani, Y., Doi, J., Itou, K., Ciucchi, B., & Pashley, D. H. (2003). Resin adhesion to carious dentin. American journal of dentistry, 16(1), 47-52.