Resistin stimulation of 17α-hydroxylase activity in ovarian theca cells in Vitro

Relevance to polycystic ovary syndrome

Iqbal Munir, Hui Wen Yen, Talia Baruth, Rafal Tarkowski, Ricardo Azziz, Denis A. Magoffin, Artur J. Jakimiuk

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Context: A newly discovered hormone resistin has been shown to be increased in women with polycystic ovary syndrome (PCOS). Objective: The purpose of this study was to confirm increased resistin concentrations in women with PCOS and to test the direct effect of resistin on human theca cell androgen production. Design: Resistin was measured in fasting serum samples by RIA. To test the direct effects of resistin on ovarian androgen biosynthesis, human theca cells were cultured with resistin for 3 d in the presence and absence of forskolin and insulin. Patients: Fasting serum samples were obtained from 45 women with PCOS and 74 regularly cycling premenopausal control women in the follicular phase of their menstrual cycles, and ovarian theca cell cultures were established from two control women. Results: The mean serum resistin concentration was increased (40%) in women with PCOS. Serum resistin concentrations correlated positively with body mass index and testosterone in PCOS women but not in controls. There were no significant correlations between resistin and fasting insulin or indicators of insulin resistance when corrected for body mass index. In cultured human theca cells, basal 17α-hydroxylase activity was unchanged by resistin alone, but resistin enhanced 17α-hydroxylase activity in the presence of forskolin or a combination of forskolin plus insulin. Resistin (≥1 ng/ml) augmented forskolin and forskolin plus insulin stimulation of CYP17 mRNA expression in a concentration-dependent manner. Conclusion: These data indicate that abnormal resistin secretion in PCOS may play a role in causing ovarian hyperandrogenism.

Original languageEnglish (US)
Pages (from-to)4852-4857
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number8
DOIs
StatePublished - Aug 1 2005

Fingerprint

Theca Cells
Resistin
Polycystic Ovary Syndrome
Mixed Function Oxygenases
Colforsin
Insulin
Fasting
Serum
Androgens
In Vitro Techniques
Body Mass Index
Steroid 17-alpha-Hydroxylase
Hyperandrogenism
Follicular Phase
Biosynthesis
Cell culture
Insulin Resistance
Testosterone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Resistin stimulation of 17α-hydroxylase activity in ovarian theca cells in Vitro : Relevance to polycystic ovary syndrome. / Munir, Iqbal; Yen, Hui Wen; Baruth, Talia; Tarkowski, Rafal; Azziz, Ricardo; Magoffin, Denis A.; Jakimiuk, Artur J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 8, 01.08.2005, p. 4852-4857.

Research output: Contribution to journalArticle

Munir, Iqbal ; Yen, Hui Wen ; Baruth, Talia ; Tarkowski, Rafal ; Azziz, Ricardo ; Magoffin, Denis A. ; Jakimiuk, Artur J. / Resistin stimulation of 17α-hydroxylase activity in ovarian theca cells in Vitro : Relevance to polycystic ovary syndrome. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 8. pp. 4852-4857.
@article{49a67b0b5e5a4970bfa8ea223425e193,
title = "Resistin stimulation of 17α-hydroxylase activity in ovarian theca cells in Vitro: Relevance to polycystic ovary syndrome",
abstract = "Context: A newly discovered hormone resistin has been shown to be increased in women with polycystic ovary syndrome (PCOS). Objective: The purpose of this study was to confirm increased resistin concentrations in women with PCOS and to test the direct effect of resistin on human theca cell androgen production. Design: Resistin was measured in fasting serum samples by RIA. To test the direct effects of resistin on ovarian androgen biosynthesis, human theca cells were cultured with resistin for 3 d in the presence and absence of forskolin and insulin. Patients: Fasting serum samples were obtained from 45 women with PCOS and 74 regularly cycling premenopausal control women in the follicular phase of their menstrual cycles, and ovarian theca cell cultures were established from two control women. Results: The mean serum resistin concentration was increased (40{\%}) in women with PCOS. Serum resistin concentrations correlated positively with body mass index and testosterone in PCOS women but not in controls. There were no significant correlations between resistin and fasting insulin or indicators of insulin resistance when corrected for body mass index. In cultured human theca cells, basal 17α-hydroxylase activity was unchanged by resistin alone, but resistin enhanced 17α-hydroxylase activity in the presence of forskolin or a combination of forskolin plus insulin. Resistin (≥1 ng/ml) augmented forskolin and forskolin plus insulin stimulation of CYP17 mRNA expression in a concentration-dependent manner. Conclusion: These data indicate that abnormal resistin secretion in PCOS may play a role in causing ovarian hyperandrogenism.",
author = "Iqbal Munir and Yen, {Hui Wen} and Talia Baruth and Rafal Tarkowski and Ricardo Azziz and Magoffin, {Denis A.} and Jakimiuk, {Artur J.}",
year = "2005",
month = "8",
day = "1",
doi = "10.1210/jc.2004-2152",
language = "English (US)",
volume = "90",
pages = "4852--4857",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "8",

}

TY - JOUR

T1 - Resistin stimulation of 17α-hydroxylase activity in ovarian theca cells in Vitro

T2 - Relevance to polycystic ovary syndrome

AU - Munir, Iqbal

AU - Yen, Hui Wen

AU - Baruth, Talia

AU - Tarkowski, Rafal

AU - Azziz, Ricardo

AU - Magoffin, Denis A.

AU - Jakimiuk, Artur J.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Context: A newly discovered hormone resistin has been shown to be increased in women with polycystic ovary syndrome (PCOS). Objective: The purpose of this study was to confirm increased resistin concentrations in women with PCOS and to test the direct effect of resistin on human theca cell androgen production. Design: Resistin was measured in fasting serum samples by RIA. To test the direct effects of resistin on ovarian androgen biosynthesis, human theca cells were cultured with resistin for 3 d in the presence and absence of forskolin and insulin. Patients: Fasting serum samples were obtained from 45 women with PCOS and 74 regularly cycling premenopausal control women in the follicular phase of their menstrual cycles, and ovarian theca cell cultures were established from two control women. Results: The mean serum resistin concentration was increased (40%) in women with PCOS. Serum resistin concentrations correlated positively with body mass index and testosterone in PCOS women but not in controls. There were no significant correlations between resistin and fasting insulin or indicators of insulin resistance when corrected for body mass index. In cultured human theca cells, basal 17α-hydroxylase activity was unchanged by resistin alone, but resistin enhanced 17α-hydroxylase activity in the presence of forskolin or a combination of forskolin plus insulin. Resistin (≥1 ng/ml) augmented forskolin and forskolin plus insulin stimulation of CYP17 mRNA expression in a concentration-dependent manner. Conclusion: These data indicate that abnormal resistin secretion in PCOS may play a role in causing ovarian hyperandrogenism.

AB - Context: A newly discovered hormone resistin has been shown to be increased in women with polycystic ovary syndrome (PCOS). Objective: The purpose of this study was to confirm increased resistin concentrations in women with PCOS and to test the direct effect of resistin on human theca cell androgen production. Design: Resistin was measured in fasting serum samples by RIA. To test the direct effects of resistin on ovarian androgen biosynthesis, human theca cells were cultured with resistin for 3 d in the presence and absence of forskolin and insulin. Patients: Fasting serum samples were obtained from 45 women with PCOS and 74 regularly cycling premenopausal control women in the follicular phase of their menstrual cycles, and ovarian theca cell cultures were established from two control women. Results: The mean serum resistin concentration was increased (40%) in women with PCOS. Serum resistin concentrations correlated positively with body mass index and testosterone in PCOS women but not in controls. There were no significant correlations between resistin and fasting insulin or indicators of insulin resistance when corrected for body mass index. In cultured human theca cells, basal 17α-hydroxylase activity was unchanged by resistin alone, but resistin enhanced 17α-hydroxylase activity in the presence of forskolin or a combination of forskolin plus insulin. Resistin (≥1 ng/ml) augmented forskolin and forskolin plus insulin stimulation of CYP17 mRNA expression in a concentration-dependent manner. Conclusion: These data indicate that abnormal resistin secretion in PCOS may play a role in causing ovarian hyperandrogenism.

UR - http://www.scopus.com/inward/record.url?scp=23844484845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844484845&partnerID=8YFLogxK

U2 - 10.1210/jc.2004-2152

DO - 10.1210/jc.2004-2152

M3 - Article

VL - 90

SP - 4852

EP - 4857

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 8

ER -