Responses of oral epithelial cells to dental resin components)

Carol A Lefebvre, G. S. Schuster, Frederick Rueggeberg, K. Tamare-Selvy, K. L. Knoernschild

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The light-polymerized resins used in dentistry and their various constituents have been shown to produce significant levels of cytotoxicity, depending upon the material and the cell type exposed to it. These responses include altered cell growth and macromolecule synthesis. The current study examined the effects of several resin components on growth and lipid metabolism of oral epithelial cells. Resin discs were fabricated from triethyleneglycol dimethacrylate (TEGDMA) as received from the manufacturer and after removal of the stabilizer methyl ether hydroquinone (MEHQ). Some discs also contained the initiators benzoyl peroxide (BPO) and camphoroquinone (CQ), and/or an activator, dimethylaminoethyl methacrylate (DMAEMA). After polymerization, the ability of components to elute from the discs and alter cell growth and lipid synthesis were assayed by a colorimetric method and thin layer chromatography respectively. Purified TEGDMA had little effect on the cells' growth or lipid metabolism, while TEGDMA containing MEHQ did inhibit growth as well as total polar lipid synthesis. Eluates from discs containing DMAEMA inhibited cell growth as well as decreasing polar lipid formation. However, this same material produced increased synthesis of diglycerides and cholesterol esters. Eluates from BPO-containing discs, as well as those with CQ, with or without DMAEMA resulted in increased levels of diglycerides. These results demonstrate that even after polymerization, components used in dental resins may elute into the immediate environment and alter various cell metabolic processes.

Original languageEnglish (US)
Pages (from-to)965-976
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume7
Issue number11
DOIs
StatePublished - Jan 1 1996

Fingerprint

Tooth Components
Synthetic Resins
Cell growth
Methacrylates
Resins
Epithelial Cells
Benzoyl Peroxide
Lipids
Benzoyl peroxide
Diglycerides
Growth
Ethers
Polymerization
Lipid Metabolism
Dentistry
Thin layer chromatography
Cholesterol Esters
Cholesterol
Cytotoxicity
Macromolecules

Keywords

  • Cell growth
  • Lipids
  • Metabolism
  • Oral epithelium
  • Resins

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Responses of oral epithelial cells to dental resin components). / Lefebvre, Carol A; Schuster, G. S.; Rueggeberg, Frederick; Tamare-Selvy, K.; Knoernschild, K. L.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 7, No. 11, 01.01.1996, p. 965-976.

Research output: Contribution to journalArticle

Lefebvre, Carol A ; Schuster, G. S. ; Rueggeberg, Frederick ; Tamare-Selvy, K. ; Knoernschild, K. L. / Responses of oral epithelial cells to dental resin components). In: Journal of Biomaterials Science, Polymer Edition. 1996 ; Vol. 7, No. 11. pp. 965-976.
@article{7835726002064393989a0e9fddd3de0c,
title = "Responses of oral epithelial cells to dental resin components)",
abstract = "The light-polymerized resins used in dentistry and their various constituents have been shown to produce significant levels of cytotoxicity, depending upon the material and the cell type exposed to it. These responses include altered cell growth and macromolecule synthesis. The current study examined the effects of several resin components on growth and lipid metabolism of oral epithelial cells. Resin discs were fabricated from triethyleneglycol dimethacrylate (TEGDMA) as received from the manufacturer and after removal of the stabilizer methyl ether hydroquinone (MEHQ). Some discs also contained the initiators benzoyl peroxide (BPO) and camphoroquinone (CQ), and/or an activator, dimethylaminoethyl methacrylate (DMAEMA). After polymerization, the ability of components to elute from the discs and alter cell growth and lipid synthesis were assayed by a colorimetric method and thin layer chromatography respectively. Purified TEGDMA had little effect on the cells' growth or lipid metabolism, while TEGDMA containing MEHQ did inhibit growth as well as total polar lipid synthesis. Eluates from discs containing DMAEMA inhibited cell growth as well as decreasing polar lipid formation. However, this same material produced increased synthesis of diglycerides and cholesterol esters. Eluates from BPO-containing discs, as well as those with CQ, with or without DMAEMA resulted in increased levels of diglycerides. These results demonstrate that even after polymerization, components used in dental resins may elute into the immediate environment and alter various cell metabolic processes.",
keywords = "Cell growth, Lipids, Metabolism, Oral epithelium, Resins",
author = "Lefebvre, {Carol A} and Schuster, {G. S.} and Frederick Rueggeberg and K. Tamare-Selvy and Knoernschild, {K. L.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1163/156856296X00372",
language = "English (US)",
volume = "7",
pages = "965--976",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "11",

}

TY - JOUR

T1 - Responses of oral epithelial cells to dental resin components)

AU - Lefebvre, Carol A

AU - Schuster, G. S.

AU - Rueggeberg, Frederick

AU - Tamare-Selvy, K.

AU - Knoernschild, K. L.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The light-polymerized resins used in dentistry and their various constituents have been shown to produce significant levels of cytotoxicity, depending upon the material and the cell type exposed to it. These responses include altered cell growth and macromolecule synthesis. The current study examined the effects of several resin components on growth and lipid metabolism of oral epithelial cells. Resin discs were fabricated from triethyleneglycol dimethacrylate (TEGDMA) as received from the manufacturer and after removal of the stabilizer methyl ether hydroquinone (MEHQ). Some discs also contained the initiators benzoyl peroxide (BPO) and camphoroquinone (CQ), and/or an activator, dimethylaminoethyl methacrylate (DMAEMA). After polymerization, the ability of components to elute from the discs and alter cell growth and lipid synthesis were assayed by a colorimetric method and thin layer chromatography respectively. Purified TEGDMA had little effect on the cells' growth or lipid metabolism, while TEGDMA containing MEHQ did inhibit growth as well as total polar lipid synthesis. Eluates from discs containing DMAEMA inhibited cell growth as well as decreasing polar lipid formation. However, this same material produced increased synthesis of diglycerides and cholesterol esters. Eluates from BPO-containing discs, as well as those with CQ, with or without DMAEMA resulted in increased levels of diglycerides. These results demonstrate that even after polymerization, components used in dental resins may elute into the immediate environment and alter various cell metabolic processes.

AB - The light-polymerized resins used in dentistry and their various constituents have been shown to produce significant levels of cytotoxicity, depending upon the material and the cell type exposed to it. These responses include altered cell growth and macromolecule synthesis. The current study examined the effects of several resin components on growth and lipid metabolism of oral epithelial cells. Resin discs were fabricated from triethyleneglycol dimethacrylate (TEGDMA) as received from the manufacturer and after removal of the stabilizer methyl ether hydroquinone (MEHQ). Some discs also contained the initiators benzoyl peroxide (BPO) and camphoroquinone (CQ), and/or an activator, dimethylaminoethyl methacrylate (DMAEMA). After polymerization, the ability of components to elute from the discs and alter cell growth and lipid synthesis were assayed by a colorimetric method and thin layer chromatography respectively. Purified TEGDMA had little effect on the cells' growth or lipid metabolism, while TEGDMA containing MEHQ did inhibit growth as well as total polar lipid synthesis. Eluates from discs containing DMAEMA inhibited cell growth as well as decreasing polar lipid formation. However, this same material produced increased synthesis of diglycerides and cholesterol esters. Eluates from BPO-containing discs, as well as those with CQ, with or without DMAEMA resulted in increased levels of diglycerides. These results demonstrate that even after polymerization, components used in dental resins may elute into the immediate environment and alter various cell metabolic processes.

KW - Cell growth

KW - Lipids

KW - Metabolism

KW - Oral epithelium

KW - Resins

UR - http://www.scopus.com/inward/record.url?scp=0029785369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029785369&partnerID=8YFLogxK

U2 - 10.1163/156856296X00372

DO - 10.1163/156856296X00372

M3 - Article

C2 - 8858485

AN - SCOPUS:0029785369

VL - 7

SP - 965

EP - 976

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 11

ER -