Restriction fragment length polymorphism analysis of the VX locus in human lupus

Moncef Zouali, Michael P. Madaio, Rosa T. Canoso, B. David Stollar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To evaluate the degree of genetic polymorphism of the Vχ; repertoire in systemic lupus erythematosus (SLE), we performed Southern blot hybridizations with human gene probes corresponding to the four human Vχ; gene families. In a comparative analysis, non‐lymphoid cell DNA samples from three patients with idiopathic SLE, eight subjects with susceptibility to drug‐induced lupus and seven control individuals were digested with the restriction endonucleases Bam HI, Bg1 II, Eco RI and Hind III, and hybridized sequentially to the four Vχ; family‐specific probes. The restriction patterns on Southern blots revealed a low degree of polymorphism of the human Vχ; gene repertoires of SLE patients and control individuals. This analysis, together with previous parallel studies of the Vχ; locus in lupus‐prone mice, implies that autoantibody hyperproduction in lupus is not associated with major modifications in the structure or genomic organization of immunoglobulin light chain genes.

Original languageEnglish (US)
Pages (from-to)1757-1760
Number of pages4
JournalEuropean Journal of Immunology
Volume19
Issue number9
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Restriction Fragment Length Polymorphisms
Systemic Lupus Erythematosus
Southern Blotting
Immunoglobulin Light Chain Genes
Deoxyribonuclease EcoRI
Genes
DNA Restriction Enzymes
Genetic Polymorphisms
Autoantibodies
DNA
VX

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Restriction fragment length polymorphism analysis of the VX locus in human lupus. / Zouali, Moncef; Madaio, Michael P.; Canoso, Rosa T.; Stollar, B. David.

In: European Journal of Immunology, Vol. 19, No. 9, 01.01.1989, p. 1757-1760.

Research output: Contribution to journalArticle

Zouali, Moncef ; Madaio, Michael P. ; Canoso, Rosa T. ; Stollar, B. David. / Restriction fragment length polymorphism analysis of the VX locus in human lupus. In: European Journal of Immunology. 1989 ; Vol. 19, No. 9. pp. 1757-1760.
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