Retina-specific expression from the IRBP promoter in transgenic mice is conferred by 212 bp of the 5′-flanking region

Gregory I Liou, Suraporn Matragoon, Jun Yang, Li Geng, Paul A. Overbeek, Din Pow Ma

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

IRBP is a photoreceptor-specific glycoprotein that has been suggested as a retinoid carrier in the visual process. Previous research has shown that 1.3 kb of 5′-flanking sequence from the human IRBP gene is sufficient to promote photoreceptor-specific expression of reporter genes in transgenic mice. To define more narrowly the sequences that promote tissue-specific expression, chimeric constructs with shorter promoters were used to generate transgenic mice. The bacterial CAT gene was fused to fragments of 706 bp or 212 bp from the 5′ end of the human IRBP gene. Analysis of the three transgenic families bearing the 706 bp IRBP promoter revealed that CAT expression was confined to the neuro-retina and the pineal gland. Analysis of the four transgenic families bearing the 212 bp IRBP promoter revealed the same tissue-specific CAT expression in three families. These results establish that tissue-specific expression of IRBP can be regulated by a short 212 bp promoter which has been conserved between humans and mice.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number1
DOIs
StatePublished - Nov 27 1991

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5' Flanking Region
Transgenic Mice
Retina
Bearings (structural)
Genes
Tissue
Bacterial Genes
Pineal Gland
Retinoids
Reporter Genes
Glycoproteins
Research

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Retina-specific expression from the IRBP promoter in transgenic mice is conferred by 212 bp of the 5′-flanking region. / Liou, Gregory I; Matragoon, Suraporn; Yang, Jun; Geng, Li; Overbeek, Paul A.; Ma, Din Pow.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 1, 27.11.1991, p. 159-165.

Research output: Contribution to journalArticle

Liou, Gregory I ; Matragoon, Suraporn ; Yang, Jun ; Geng, Li ; Overbeek, Paul A. ; Ma, Din Pow. / Retina-specific expression from the IRBP promoter in transgenic mice is conferred by 212 bp of the 5′-flanking region. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 1. pp. 159-165.
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