Reversal by human chorionic gonadotropin of the inhibitory effect of clomiphene on progesterone production by granulosa-luteal cells in culture

G. Lavy, Michael Peter Diamond, M. L. Polan

Research output: Contribution to journalArticle

Abstract

The possibility of a direct ovarian effect of clomiphene citrate was assessed in vitro by measuring progesterone release from cultured human granulosa-lutheal cells in both the presence and absence of hCG. Granulosa-luteal cells obtained by laparoscopic follicle aspiration following ovarian stimulation with hMG and hCG in women undergoing in vitro fertilization/embryo transfer were incubated for 72 hours in medium containing increasing concentrations of clomiphene (10-9-10-5 M) both without and with hCG (1 IU/mL). Clomiphene stimulated progesterone production in both the presence and absence of additional hCG at concentrations ≤ 107 M. In both the presence and absence of hCG a dose-dependent inhibition of progesterone production was observed with higher concentrations of clomiphene (> 106 M). hCG (1 IU/mL) significantly increased progesterone production in control cells and at all concentrations of clomiphene (10-9-10-5 M) tested. Low clomiphene (10-9-10-7 M) and hCG concentrations appeared to have a synergistic effect on progesterone production. Thus it appears that clomiphene has a direct ovarian effect on progesterone production, which is stimulatory at low concentrations and inhibitory at higher concentrations.

Original languageEnglish (US)
Pages (from-to)359-362
Number of pages4
JournalInternational Journal of Fertility
Volume34
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes

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Luteal Cells
Clomiphene
Chorionic Gonadotropin
Progesterone
Cell Culture Techniques
Ovulation Induction
Granulosa Cells
Embryo Transfer
Fertilization in Vitro

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

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abstract = "The possibility of a direct ovarian effect of clomiphene citrate was assessed in vitro by measuring progesterone release from cultured human granulosa-lutheal cells in both the presence and absence of hCG. Granulosa-luteal cells obtained by laparoscopic follicle aspiration following ovarian stimulation with hMG and hCG in women undergoing in vitro fertilization/embryo transfer were incubated for 72 hours in medium containing increasing concentrations of clomiphene (10-9-10-5 M) both without and with hCG (1 IU/mL). Clomiphene stimulated progesterone production in both the presence and absence of additional hCG at concentrations ≤ 107 M. In both the presence and absence of hCG a dose-dependent inhibition of progesterone production was observed with higher concentrations of clomiphene (> 106 M). hCG (1 IU/mL) significantly increased progesterone production in control cells and at all concentrations of clomiphene (10-9-10-5 M) tested. Low clomiphene (10-9-10-7 M) and hCG concentrations appeared to have a synergistic effect on progesterone production. Thus it appears that clomiphene has a direct ovarian effect on progesterone production, which is stimulatory at low concentrations and inhibitory at higher concentrations.",
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AU - Polan, M. L.

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