Ridge Augmentation Following Implantation of Recombinant Human Bone Morphogenetic Protein-2 in the Dog

Eliane Porto Barboza, Maria Eugênia Leite Duarte, Luiz Geolás, Rachel G. Sorensen, Gerard E. Riedel, Ulf M E Wikesjö

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier induces bone for reconstruction of skeletal defects. The rhBMP-2/ACS implant is prepared by administering a rhBMP-2 solution to dry ACS. Once prepared, rhBMP-2/ACS forms a moldable, cohesive, and adhesive implant. However, rhBMP-2/ACS does not have sufficient structural strength to withstand soft tissue compression at specific anatomic sites. To more fully understand the mechanisms that affect bone induction by rhBMP-2/ACS in the presence of soft tissue compression, it would be useful to have a preclinical model that appropriately simulates such circumstances in patients. This pilot study evaluated one such potential model. Methods: Bilateral, Class III alveolar defects were surgically produced in 4 adult mongrel dogs following extraction of the mandibular fourth premolars and reduction of the alveolar ridge. After an 8-week healing interval, mucoperiosteal flaps were elevated and rhBMP-2/ACS or rhBMP-2/ ACS combined with hydroxyapatite (HA) was implanted into contralateral defects. The animals were euthanized at 12 weeks post-augmentation and block biopsies processed for histologic evaluation. Results: Limited augmentation followed implantation of rhBMP-2/ACS (0.7 ± 0.6 mm). In contrast, sites receiving rhBMP-2/ACS/HA exhibited clinically relevant ridge augmentation (5.5 ± 1.6 mm). Defects implanted with rhBMP-2/ACS exhibited dense trabeculation within the corpus of the reduced alveolar process. The cortices appeared intact without evidence of expansion into the defect area. Three defects receiving rhBMP-2/ ACS/HA exhibited sparse bone trabeculae amidst HA particles, fibrovascular tissue, and marrow. Commonly, the HA particles were encapsulated by fibrous tissue. Some particles were observed in contact with bone. Conclusions: The results suggests that rhBMP-2/ACS has limited effect alone in this augmentation model of Class III alveolar ridge defects. Inclusion of HA into the rhBMP-2 construct results in clinically relevant augmentation, however, the quality of bone is compromised.

Original languageEnglish (US)
Pages (from-to)488-496
Number of pages9
JournalJournal of Periodontology
Volume71
Issue number3
DOIs
StatePublished - Jan 1 2000

Fingerprint

Porifera
Collagen
Dogs
Durapatite
Alveolar Process
Bone and Bones
recombinant human bone morphogenetic protein-2
Bicuspid
Adhesives
Bone Marrow

Keywords

  • Alveolar ridge augmentation
  • Animal studies
  • Bone morphogenetic proteins, recombinant
  • Bone regeneration
  • Hydroxyapatite/therapeutic use
  • Wound healing

ASJC Scopus subject areas

  • Periodontics

Cite this

Barboza, E. P., Duarte, M. E. L., Geolás, L., Sorensen, R. G., Riedel, G. E., & Wikesjö, U. M. E. (2000). Ridge Augmentation Following Implantation of Recombinant Human Bone Morphogenetic Protein-2 in the Dog. Journal of Periodontology, 71(3), 488-496. https://doi.org/10.1902/jop.2000.71.3.488

Ridge Augmentation Following Implantation of Recombinant Human Bone Morphogenetic Protein-2 in the Dog. / Barboza, Eliane Porto; Duarte, Maria Eugênia Leite; Geolás, Luiz; Sorensen, Rachel G.; Riedel, Gerard E.; Wikesjö, Ulf M E.

In: Journal of Periodontology, Vol. 71, No. 3, 01.01.2000, p. 488-496.

Research output: Contribution to journalArticle

Barboza, EP, Duarte, MEL, Geolás, L, Sorensen, RG, Riedel, GE & Wikesjö, UME 2000, 'Ridge Augmentation Following Implantation of Recombinant Human Bone Morphogenetic Protein-2 in the Dog', Journal of Periodontology, vol. 71, no. 3, pp. 488-496. https://doi.org/10.1902/jop.2000.71.3.488
Barboza, Eliane Porto ; Duarte, Maria Eugênia Leite ; Geolás, Luiz ; Sorensen, Rachel G. ; Riedel, Gerard E. ; Wikesjö, Ulf M E. / Ridge Augmentation Following Implantation of Recombinant Human Bone Morphogenetic Protein-2 in the Dog. In: Journal of Periodontology. 2000 ; Vol. 71, No. 3. pp. 488-496.
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abstract = "Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier induces bone for reconstruction of skeletal defects. The rhBMP-2/ACS implant is prepared by administering a rhBMP-2 solution to dry ACS. Once prepared, rhBMP-2/ACS forms a moldable, cohesive, and adhesive implant. However, rhBMP-2/ACS does not have sufficient structural strength to withstand soft tissue compression at specific anatomic sites. To more fully understand the mechanisms that affect bone induction by rhBMP-2/ACS in the presence of soft tissue compression, it would be useful to have a preclinical model that appropriately simulates such circumstances in patients. This pilot study evaluated one such potential model. Methods: Bilateral, Class III alveolar defects were surgically produced in 4 adult mongrel dogs following extraction of the mandibular fourth premolars and reduction of the alveolar ridge. After an 8-week healing interval, mucoperiosteal flaps were elevated and rhBMP-2/ACS or rhBMP-2/ ACS combined with hydroxyapatite (HA) was implanted into contralateral defects. The animals were euthanized at 12 weeks post-augmentation and block biopsies processed for histologic evaluation. Results: Limited augmentation followed implantation of rhBMP-2/ACS (0.7 ± 0.6 mm). In contrast, sites receiving rhBMP-2/ACS/HA exhibited clinically relevant ridge augmentation (5.5 ± 1.6 mm). Defects implanted with rhBMP-2/ACS exhibited dense trabeculation within the corpus of the reduced alveolar process. The cortices appeared intact without evidence of expansion into the defect area. Three defects receiving rhBMP-2/ ACS/HA exhibited sparse bone trabeculae amidst HA particles, fibrovascular tissue, and marrow. Commonly, the HA particles were encapsulated by fibrous tissue. Some particles were observed in contact with bone. Conclusions: The results suggests that rhBMP-2/ACS has limited effect alone in this augmentation model of Class III alveolar ridge defects. Inclusion of HA into the rhBMP-2 construct results in clinically relevant augmentation, however, the quality of bone is compromised.",
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AU - Riedel, Gerard E.

AU - Wikesjö, Ulf M E

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N2 - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier induces bone for reconstruction of skeletal defects. The rhBMP-2/ACS implant is prepared by administering a rhBMP-2 solution to dry ACS. Once prepared, rhBMP-2/ACS forms a moldable, cohesive, and adhesive implant. However, rhBMP-2/ACS does not have sufficient structural strength to withstand soft tissue compression at specific anatomic sites. To more fully understand the mechanisms that affect bone induction by rhBMP-2/ACS in the presence of soft tissue compression, it would be useful to have a preclinical model that appropriately simulates such circumstances in patients. This pilot study evaluated one such potential model. Methods: Bilateral, Class III alveolar defects were surgically produced in 4 adult mongrel dogs following extraction of the mandibular fourth premolars and reduction of the alveolar ridge. After an 8-week healing interval, mucoperiosteal flaps were elevated and rhBMP-2/ACS or rhBMP-2/ ACS combined with hydroxyapatite (HA) was implanted into contralateral defects. The animals were euthanized at 12 weeks post-augmentation and block biopsies processed for histologic evaluation. Results: Limited augmentation followed implantation of rhBMP-2/ACS (0.7 ± 0.6 mm). In contrast, sites receiving rhBMP-2/ACS/HA exhibited clinically relevant ridge augmentation (5.5 ± 1.6 mm). Defects implanted with rhBMP-2/ACS exhibited dense trabeculation within the corpus of the reduced alveolar process. The cortices appeared intact without evidence of expansion into the defect area. Three defects receiving rhBMP-2/ ACS/HA exhibited sparse bone trabeculae amidst HA particles, fibrovascular tissue, and marrow. Commonly, the HA particles were encapsulated by fibrous tissue. Some particles were observed in contact with bone. Conclusions: The results suggests that rhBMP-2/ACS has limited effect alone in this augmentation model of Class III alveolar ridge defects. Inclusion of HA into the rhBMP-2 construct results in clinically relevant augmentation, however, the quality of bone is compromised.

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