RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana

Xiao Bao Ying, Li Dong, Hui Zhu, Cheng Guo Duan, Quan Sheng Du, Dian Qiu Lv, Yuan Yuan Fang, Juan Antonio Garcia, Rong Xiang Fang, Hui Shan Guo

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Endogenous eukaryotic RNA-dependent RNA polymerases (RDRs) produce double-stranded RNA intermediates in diverse processes of small RNA synthesis in RNA silencing pathways. RDR6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (S-PTGS) and has an important role in amplification of antiviral silencing. Whereas RDR1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. In this study, we show that Nicotiana benthamiana transformed with RDR1 from Nicotiana tabacum (Nt-RDR1 plants) exhibits hypersusceptibility to Plum pox potyvirus and other viruses, resembling RDR6-silenced (RDR6i) N. benthamiana. Analysis of transient induction of RNA silencing in N. benthamiana Nt-RDR1 and RDR6i plants revealed that Nt-RDR1 possesses silencing suppression activity. We found that Nt-RDR1 does not interfere with RDR6-dependent siRNA accumulation but turns out to suppress RDR6-dependent S-PTGS. Our results, together with previously published data, suggest that RDR1 might have a dual role, contributing, on one hand, to salicylic acid-mediated antiviral defense, and suppressing, on the other hand, the RDR6-mediated antiviral RNA silencing. We propose a scenario in which the natural loss-of-function variant of RDR1 in N. benthamiana may be the outcome of selective pressure to maintain a high RDR6-dependent antiviral defense, which would be required to face the hypersensitivity of this plant to a large number of viruses.

Original languageEnglish (US)
Pages (from-to)1358-1372
Number of pages15
JournalPlant Cell
Volume22
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

RNA-directed RNA polymerase
RNA Replicase
Nicotiana benthamiana
Virus Diseases
RNA Interference
RNA interference
Nicotiana tabacum
Tobacco
Antiviral Agents
infection
Plum pox virus
viruses
Potyvirus
double-stranded RNA
Viruses
hypersensitive response
small interfering RNA
Plant Genes
salicylic acid
Salicylic Acid

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. / Ying, Xiao Bao; Dong, Li; Zhu, Hui; Duan, Cheng Guo; Du, Quan Sheng; Lv, Dian Qiu; Fang, Yuan Yuan; Garcia, Juan Antonio; Fang, Rong Xiang; Guo, Hui Shan.

In: Plant Cell, Vol. 22, No. 4, 01.04.2010, p. 1358-1372.

Research output: Contribution to journalArticle

Ying, XB, Dong, L, Zhu, H, Duan, CG, Du, QS, Lv, DQ, Fang, YY, Garcia, JA, Fang, RX & Guo, HS 2010, 'RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana', Plant Cell, vol. 22, no. 4, pp. 1358-1372. https://doi.org/10.1105/tpc.109.072058
Ying, Xiao Bao ; Dong, Li ; Zhu, Hui ; Duan, Cheng Guo ; Du, Quan Sheng ; Lv, Dian Qiu ; Fang, Yuan Yuan ; Garcia, Juan Antonio ; Fang, Rong Xiang ; Guo, Hui Shan. / RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. In: Plant Cell. 2010 ; Vol. 22, No. 4. pp. 1358-1372.
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AU - Du, Quan Sheng

AU - Lv, Dian Qiu

AU - Fang, Yuan Yuan

AU - Garcia, Juan Antonio

AU - Fang, Rong Xiang

AU - Guo, Hui Shan

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