RNA localization in yeast: Moving towards a mechanism

Graydon B. Gonsalvez, Carl R. Urbinati, Roy M. Long

Research output: Contribution to journalReview article

89 Citations (Scopus)

Abstract

RNA localization is a widely utilized strategy employed by cells to spatially restrict protein function. In Saccharomyces cerevisiae asymmetric sorting of mRNA to the bud has been reported for at least 24 mRNAs. The mechanism by which the mRNAs are trafficked to the bud, illustrated by ASH1 mRNA, involves recognition of cis-acting localization elements present in the mRNA by the RNA-binding protein, She2p. The She2p/mRNA complex subsequently associates with the myosin motor protein, Myo4p, through an adapter, She3p. This ribonucleoprotein complex is transported to the distal tip of the bud along polarized actin cables. While the mechanism by which ASM mRNA is anchored at the bud tip is unknown, current data point to a role for translation in this process, and the rate of translation of Ash1p during the transport phase is regulated by the cis-acting localization elements. Subcellular sorting of mRNA in yeast is not limited to the bud; certain mRNAs corresponding to nuclear-encoded mitochondrial proteins are specifically sorted to the proximity of mitochondria. Analogous to ASH1 mRNA localization, mitochondrial sorting requires cis-acting elements present in the mRNA, though trans-acting factors involved with this process remain to be identified. This review aims to discuss mechanistic details of mRNA localization in S. cerevisia.

Original languageEnglish (US)
Pages (from-to)75-86
Number of pages12
JournalBiology of the Cell
Volume97
Issue number1
DOIs
StatePublished - Jan 1 2005

Fingerprint

Yeasts
RNA
Messenger RNA
RNA Transport
Saccharomyces cerevisiae
Ribonucleoproteins
Trans-Activators
RNA-Binding Proteins
Mitochondrial Proteins
Myosins
Actins
Mitochondria
Proteins

Keywords

  • ASH1
  • Anchoring
  • Polarization
  • RNP
  • Transport

ASJC Scopus subject areas

  • Cell Biology

Cite this

RNA localization in yeast : Moving towards a mechanism. / Gonsalvez, Graydon B.; Urbinati, Carl R.; Long, Roy M.

In: Biology of the Cell, Vol. 97, No. 1, 01.01.2005, p. 75-86.

Research output: Contribution to journalReview article

Gonsalvez, Graydon B. ; Urbinati, Carl R. ; Long, Roy M. / RNA localization in yeast : Moving towards a mechanism. In: Biology of the Cell. 2005 ; Vol. 97, No. 1. pp. 75-86.
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