RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins

Samantha H. Chiang, Gerald A. Thomas, Wei Liao, Tristan Grogan, Robert L. Buck, Laurel Fuentes, Maha Yakob, Mary J. Laughlin, Chris Schafer, Abu Nasir Mohammad Nazmul Hossain, Fang Wei, David Elashoff, Paul D. Slowey, David T.W. Wong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The stabilization and processing of salivary transcriptome and proteome biomarkers is a critical challenge due to the ubiquitous nature of nucleases and proteases as well as the inherent instability of these biomarkers. Furthermore, extension of salivary transcriptome and proteome analysis to point-of-care and remote sites requires the availability of self-administered ambient temperature collection and storage tools. To address these challenges, a self-contained whole saliva collection and extraction system, RNAPro•SAL, has been developed that provides rapid ambient temperature collection along with concurrent processing and stabilization of extracellular RNA (exRNA) and proteins. The system was compared to the University of California, Los Angeles (UCLA) standard clinical collection process (standard operating procedure, SOP). Both systems measured total RNA and protein, and exRNA IL-8, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin and ribosomal protein S9 (RPS9) by qPCR. Proteome analysis was measured by EIA analysis of interleukin-8 (IL-8), and β-actin, as well as total protein. Over 97% of viable cells were removed by both methods. The system compared favorably to the labor-intensive clinical SOP, which requires low-temperature collection and isolation, yielding samples with similar protein and exRNA recovery and stability.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalBioTechniques
Volume58
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Saliva
Proteome
RNA
Equipment and Supplies
Biomarkers
Interleukin-8
Temperature
Actins
Proteins
Stabilization
Point-of-Care Systems
Glyceraldehyde-3-Phosphate Dehydrogenases
Los Angeles
RNA Stability
Gene Expression Profiling
Processing
Transcriptome
Peptide Hydrolases
Availability
Personnel

Keywords

  • Ambient temperature
  • Point-of-care settings
  • Saliva diagnostic tool
  • Salivary extracellular RNA
  • Salivary protein

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chiang, S. H., Thomas, G. A., Liao, W., Grogan, T., Buck, R. L., Fuentes, L., ... Wong, D. T. W. (2015). RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins. BioTechniques, 58(2), 69-76. https://doi.org/10.2144/000114254

RNAPro•SAL : A device for rapid and standardized collection of saliva RNA and proteins. / Chiang, Samantha H.; Thomas, Gerald A.; Liao, Wei; Grogan, Tristan; Buck, Robert L.; Fuentes, Laurel; Yakob, Maha; Laughlin, Mary J.; Schafer, Chris; Nazmul Hossain, Abu Nasir Mohammad; Wei, Fang; Elashoff, David; Slowey, Paul D.; Wong, David T.W.

In: BioTechniques, Vol. 58, No. 2, 01.01.2015, p. 69-76.

Research output: Contribution to journalArticle

Chiang, SH, Thomas, GA, Liao, W, Grogan, T, Buck, RL, Fuentes, L, Yakob, M, Laughlin, MJ, Schafer, C, Nazmul Hossain, ANM, Wei, F, Elashoff, D, Slowey, PD & Wong, DTW 2015, 'RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins', BioTechniques, vol. 58, no. 2, pp. 69-76. https://doi.org/10.2144/000114254
Chiang SH, Thomas GA, Liao W, Grogan T, Buck RL, Fuentes L et al. RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins. BioTechniques. 2015 Jan 1;58(2):69-76. https://doi.org/10.2144/000114254
Chiang, Samantha H. ; Thomas, Gerald A. ; Liao, Wei ; Grogan, Tristan ; Buck, Robert L. ; Fuentes, Laurel ; Yakob, Maha ; Laughlin, Mary J. ; Schafer, Chris ; Nazmul Hossain, Abu Nasir Mohammad ; Wei, Fang ; Elashoff, David ; Slowey, Paul D. ; Wong, David T.W. / RNAPro•SAL : A device for rapid and standardized collection of saliva RNA and proteins. In: BioTechniques. 2015 ; Vol. 58, No. 2. pp. 69-76.
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