Role for a YY1-binding element in replication-dependent mouse histone gene expression

Katherine A. Eliassen, Amy Baldwin, Eric M. Sikorski, Myra M. Hurt

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Expression of the highly conserved replication-dependent historic gene family increases dramatically as a cell enters the S phase of the eukaryotic cell cycle. Requirements for normal histone gene expression in vivo include an element, designated α, located within the protein-encoding sequence of nucleosomal histone genes. Mutation of 5 of 7 nucleotides of the mouse H3.2 α element to yield the sequence found in an H3.3 replication-independent variant abolishes the DNA-protein interaction in vitro and reduces expression fourfold in vivo. A yeast one-hybrid screen of a HeLa cell cDNA library identified the protein responsible for recognition of the histone H3.2 α sequence as the transcription factor Yin Yang 1 (YY1). YY1 is a ubiquitous and highly conserved transcription factor reported to be involved in both activation and repression of gene expression. Here we report that the in vitro histone α DNA-protein interaction depends on YY1 and that mutation of the nucleotides required for the in vitro histone α DNA-YY1 interaction alters the cell cycle phase-specific up-regulation of the mouse H3.2 gene in vivo. Because all mutations or deletions of the histone α sequence both abolish interactions in vitro and cause an in vivo decrease in histone gene expression, the recognition of the historic α element by YY1 is implicated in the correct temporal regulation of replication-dependent histone gene expression in vivo.

Original languageEnglish (US)
Pages (from-to)7106-7118
Number of pages13
JournalMolecular and Cellular Biology
Volume18
Issue number12
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Yin-Yang
Histones
Gene Expression
Sequence Deletion
DNA
Cell Cycle
Proteins
Transcription Factors
Nucleotides
Genes
Mutation
Eukaryotic Cells
Gene Library
S Phase
HeLa Cells
Up-Regulation
Yeasts
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Role for a YY1-binding element in replication-dependent mouse histone gene expression. / Eliassen, Katherine A.; Baldwin, Amy; Sikorski, Eric M.; Hurt, Myra M.

In: Molecular and Cellular Biology, Vol. 18, No. 12, 01.01.1998, p. 7106-7118.

Research output: Contribution to journalArticle

Eliassen, Katherine A. ; Baldwin, Amy ; Sikorski, Eric M. ; Hurt, Myra M. / Role for a YY1-binding element in replication-dependent mouse histone gene expression. In: Molecular and Cellular Biology. 1998 ; Vol. 18, No. 12. pp. 7106-7118.
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