Role for recombinant γ-glutamyltransferase from Treponema denticola in glutathione metabolism

Lianrui Chu, Xiaoping Xu, Zheng Dong, David Cappelli, Jefferey L. Ebersole

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Volatile sulfur compounds, including hydrogen sulfide (H2S), have been implicated in the development of periodontal disease. Glutathione is an important thiol source for H2S production in periodontal pockets. Our recent studies have delineated a pathway of glutathione metabolism in Treponema denticola that releases H2S. In this pathway, γ-glutamyltransferase (GGT) has been proposed to catalyze the first step of glutathione degradation. We have cloned the gene of GGT from T. denticola, which contains an open reading frame of 726 bp encoding a protein of 241 amino acids. Transformation of this gene into Escherichia coli led to the expression of a recombinant protein. After purification by chromatography, the recombinant protein showed enzymatic activity typical of GGT, catalyzing the degradation of Na-γ-glutamyl-4-nitroaniline (GNA) and the hydrolysis of glutathione, releasing glutamic acid or glutamine and cysteinylglycine. L-Cysteine is not a substrate of GGT. Importantly, GNA, when added to T. denticola, was able to compete with glutathione and inhibit the production of H2S, ammonia, and pyruvate. This was accompanied by the suppression of hemoxidative and hemolytic activities of the bacteria. Purified GGT was inactivated by TLCK (Nα-p-tosyl-L-lysine chloromethyl ketone) and proteinase K treatment. However, higher enzymatic activity was demonstrated in the presence of 2-mercaptoethanol and dithiothreitol. Our further experiments showed that the addition of recombinant GGT to Porphyromonas gingivalis, a bacterium without significant glutathione-metabolizing capacity, drastically increased the utilization of glutathione by the bacterium, producing H2S, ammonia, and pyruvate. This was again accompanied by enhanced bacterial hemoxidative and hemolytic activities. Together, the results suggest an important role for GGT in glutathione metabolism in oral bacteria.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalInfection and Immunity
Volume71
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Treponema denticola
Glutathione
Bacteria
Pyruvic Acid
Ammonia
Recombinant Proteins
cysteinylglycine
Tosyllysine Chloromethyl Ketone
Periodontal Pocket
Sulfur Compounds
Endopeptidase K
Porphyromonas gingivalis
Hydrogen Sulfide
Mercaptoethanol
Dithiothreitol
Periodontal Diseases
Glutamine
Sulfhydryl Compounds
Open Reading Frames
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Role for recombinant γ-glutamyltransferase from Treponema denticola in glutathione metabolism. / Chu, Lianrui; Xu, Xiaoping; Dong, Zheng; Cappelli, David; Ebersole, Jefferey L.

In: Infection and Immunity, Vol. 71, No. 1, 01.01.2003, p. 335-342.

Research output: Contribution to journalArticle

Chu, Lianrui ; Xu, Xiaoping ; Dong, Zheng ; Cappelli, David ; Ebersole, Jefferey L. / Role for recombinant γ-glutamyltransferase from Treponema denticola in glutathione metabolism. In: Infection and Immunity. 2003 ; Vol. 71, No. 1. pp. 335-342.
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