Role of caspase in CD80 expression of superantigen-stimulated monocytes.

Fumiaki Shinohara, Keiko Sato, Maiko Suzuki, Haruhiko Takada, Seishi Echigo, Hidemi Rikiishi

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this study, we demonstrated evidence for the induction of CD80+ monocytes by staphylococcal enterotoxin B (SEB) via caspase actions. Pre-treatment with pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of NF-kappaB, resulted in a significant reduction in the percentage of SEB- and interferon-gamma (IFN-gamma) (produced by SEB) -induced CD80+ monocytes. SEB and IFN-gamma activated NF-kappaB, which was inhibited by PDTC. SEB induced the activation of caspase-3 and -8, and pre-treatment with z-VAD-fmk, a broad-spectrum inhibitor of caspases, prevented the induction of apoptosis. Treating with z-VAD-fmk resulted in a reduction of the generation of CD80+ monocytes. These results indicated that CD80 driven by NF-kappaB is regulated by the enzymatic actions of caspases, which allows monocytes to participate in massive T-cell activation.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalInternational journal of molecular medicine
Volume14
Issue number2
StatePublished - Jan 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Shinohara, F., Sato, K., Suzuki, M., Takada, H., Echigo, S., & Rikiishi, H. (2004). Role of caspase in CD80 expression of superantigen-stimulated monocytes. International journal of molecular medicine, 14(2), 241-246.