Role of CPI-17 in the regulation of endothelial cytoskeleton

Irina A. Kolosova, Shwu Fan Ma, Djanybek M. Adyshev, Peyi Wang, Motoi Ohba, Viswanathan Natarajan, Joe G.N. Garcia, Alexander D. Verin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We have previously shown that myosin light chain (MLC) phosphatase (MLCP) is critically involved in the regulation of agonist-mediated endothelial permeability and cytoskeletal organization (Verin AD, Patterson CE, Day MA, and Garcia JG. Am J Physiol Lung Cell Mol Physiol 269: L99-L108, 1995). The molecular mechanisms of endothelial MLCP regulation, however, are not completely understood. In this study we found that, similar to smooth muscle, lung microvascular endothelial cells expressed specific endogenous inhibitor of MLCP, CPI-17. To elucidate the role of CPI-17 in the regulation of endothelial cytoskeleton, full-length CPI-17 plasmid was transiently transfected into pulmonary artery endothelial cells, where the background of endogenous protein is low. CPI-17 had no effect on cytoskeleton under nonstimulating conditions. However, stimulation of transfected cells with direct PKC activator PMA caused a dramatic increase in F-actin stress fibers, focal adhesions, and MLC phosphorylation compared with untransfected cells. Inflammatory agonist histamine and, to a much lesser extent, thrombin were capable of activating CPI-17. Histamine caused stronger CPI-17 phosphorylation than thrombin. Inhibitory analysis revealed that PKC more significantly contributes to agonist-induced CPI-17 phosphorylation than Rho-kinase. Dominant-negative PKC-α abolished the effect of CPI-17 on actin cytoskeleton, suggesting that the PKC-α isoform is most likely responsible for CPI-17 activation in the endothelium. Depletion of endogenous CPI-17 in lung microvascular endothelial cell significantly attenuated histamine-induced increase in endothelial permeability. Together these data suggest the potential importance of PKC/CPI-17-mediated pathway in histamine-triggered cytoskeletal rearrangements leading to lung microvascular barrier compromise.

Original languageEnglish (US)
Pages (from-to)L970-L980
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number5 31-5
DOIs
StatePublished - Nov 1 2004

Fingerprint

Cytoskeleton
Histamine
Lung
Endothelial Cells
Phosphorylation
Phosphoric Monoester Hydrolases
Thrombin
Permeability
cyclopropapyrroloindole
Myosin-Light-Chain Phosphatase
Histamine Agonists
rho-Associated Kinases
Stress Fibers
Myosin Light Chains
Focal Adhesions
Actin Cytoskeleton
Pulmonary Artery
Endothelium
Smooth Muscle
Actins

Keywords

  • Endothelial barrier
  • Myosin light chain phosphorylation
  • Myosin phosphatase

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Role of CPI-17 in the regulation of endothelial cytoskeleton. / Kolosova, Irina A.; Ma, Shwu Fan; Adyshev, Djanybek M.; Wang, Peyi; Ohba, Motoi; Natarajan, Viswanathan; Garcia, Joe G.N.; Verin, Alexander D.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 287, No. 5 31-5, 01.11.2004, p. L970-L980.

Research output: Contribution to journalArticle

Kolosova, IA, Ma, SF, Adyshev, DM, Wang, P, Ohba, M, Natarajan, V, Garcia, JGN & Verin, AD 2004, 'Role of CPI-17 in the regulation of endothelial cytoskeleton', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 287, no. 5 31-5, pp. L970-L980. https://doi.org/10.1152/ajplung.00398.2003
Kolosova, Irina A. ; Ma, Shwu Fan ; Adyshev, Djanybek M. ; Wang, Peyi ; Ohba, Motoi ; Natarajan, Viswanathan ; Garcia, Joe G.N. ; Verin, Alexander D. / Role of CPI-17 in the regulation of endothelial cytoskeleton. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; Vol. 287, No. 5 31-5. pp. L970-L980.
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