Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells

Brinda Bhatt, Huabin Zhu, Nikhil Patel, Nagendra Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Innate lymphoid cells (ILCs) are a recently identified family of immune cells mostly present at barrier surfaces. They play an important role in the induction, regulation, and resolution of inflammatory responses. Environmental signals play an important role in development and function of ILCs. G-protein coupled receptors (GPCRs) sense and mediate cellular responses to the environmental signals. ILCs express several G-protein coupled receptors, which play a critical role in migration of these cells to appropriate sites. Here, we describe a method to test the migration of ILCs toward 7α,25-hydroxycholesterol, which is mediated by cell surface-expressed GPR183. A similar strategy can be employed to test the role of other GPCRs in mediating the migration of ILCs toward other chemotactic ligands.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages93-98
Number of pages6
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2121
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • G-protein coupled receptors (GPCRs)
  • Innate lymphoid cells (ILCs)
  • Intestine
  • Migration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Bhatt, B., Zhu, H., Patel, N., & Singh, N. (2020). Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells. In Methods in Molecular Biology (pp. 93-98). (Methods in Molecular Biology; Vol. 2121). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0338-3_9