Role of intracellular calcium in the angiotensin II-mediated tyrosine phosphorylation and dephosphorylation of PLC-γl

J. B. Harp, P. P. Sayeski, M. Scanlon, K. E. Bernstein, Mario B Marrero

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Angiotensin II induces the rapid temporal tyrosine phosphorylation and activation of phospholipase C-γ1 (PLC-γl) and the elevation of intracellular calcium levels. To investigate the relationship of these intracellular signaling events, rat aortic smooth muscle cells were treated with the calcium chelator BAPTA-AM, the calcium channel blocker verapamil, the intracellular calcium antagonist TMB-8, and the calcium ionophore ionomycin. The effects of these agents on PLC-γl tyrosine phosphorylation were then measured. We found that treatment of these cells with the calcium inhibitors augmented the basal level of PLC-γl tyrosine phosphorylation, without changing the peak level of tyrosine phosphorylation induced by angiotensin II. The rapid dephosphorylation of PLC-γ1 that follows angiotensin II stimulation was prevented by these calcium antagonists. In contrast, angiotensin II-induced tyrosine phosphorylation of PLC-γl was inhibited by ionomycin. These results suggest that the angiotensin II-induced tyrosine phosphorylation of PLC-γ1 is calcium-independent, while the dephosphorylation is calcium-dependent.

Original languageEnglish (US)
Pages (from-to)540-544
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume232
Issue number2
DOIs
StatePublished - Mar 17 1997

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Phosphorylation
Programmable logic controllers
Angiotensin II
Tyrosine
Calcium
Ionomycin
Cells
Calcium Ionophores
Calcium Channel Blockers
Type C Phospholipases
Verapamil
Smooth Muscle Myocytes
Muscle
Rats
Chemical activation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Role of intracellular calcium in the angiotensin II-mediated tyrosine phosphorylation and dephosphorylation of PLC-γl. / Harp, J. B.; Sayeski, P. P.; Scanlon, M.; Bernstein, K. E.; Marrero, Mario B.

In: Biochemical and Biophysical Research Communications, Vol. 232, No. 2, 17.03.1997, p. 540-544.

Research output: Contribution to journalArticle

Harp, J. B. ; Sayeski, P. P. ; Scanlon, M. ; Bernstein, K. E. ; Marrero, Mario B. / Role of intracellular calcium in the angiotensin II-mediated tyrosine phosphorylation and dephosphorylation of PLC-γl. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 232, No. 2. pp. 540-544.
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