Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response

Michiko Shimoda, Tao Li, Jeanene P.S. Pihkala, Pandelakis Koni

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We investigated the role of B cell Ag presentation in homeostasis of the memory B cell compartment in a mouse model where a conditional allele for the β-chain of MHC class II (MHC-II) is deleted in the vast majority of all B cells by cd19 promoter-mediated expression of Cre recombinase (IA-B mice). Upon T cell-dependent immunization, a small number of MHC-II+ B cells in IA-B mice dramatically expanded and restored normal albeit delayed levels of germinal center (GC) B cells with an affinity-enhancing somatic mutation to Ag. IA-B mice also established normal levels of MHC-II+ memory B cells, which, however, subsequently lost MHC-II expression by ongoing deletion of the conditional iab allele without significant loss in their number. Furthermore, in vivo Ag restimulation of MHC-II- memory B cells of IA-B mice failed to cause differentiation into plasma cells (PCs), even in the presence of Ag-specific CD4+ T cells. In addition, both numbers and Ag-specific affinity of long-lived PCs during the late post-GC phase, as well as post-GC serum affinity maturation, were significantly reduced in IA-B mice. These results support a notion that MHC-II-dependent T cell help during post-GC phase is not absolutely required for the maintenance of memory B cell frequency but is important for their differentiation into PCs and for the establishment of the long-lived PC compartment.

Original languageEnglish (US)
Pages (from-to)2122-2133
Number of pages12
JournalJournal of Immunology
Volume176
Issue number4
DOIs
StatePublished - Feb 15 2006

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Germinal Center
Homeostasis
B-Lymphocytes
Plasma Cells
T-Lymphocytes
Alleles
Immunization
Maintenance
Mutation
Serum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response. / Shimoda, Michiko; Li, Tao; Pihkala, Jeanene P.S.; Koni, Pandelakis.

In: Journal of Immunology, Vol. 176, No. 4, 15.02.2006, p. 2122-2133.

Research output: Contribution to journalArticle

Shimoda, Michiko ; Li, Tao ; Pihkala, Jeanene P.S. ; Koni, Pandelakis. / Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response. In: Journal of Immunology. 2006 ; Vol. 176, No. 4. pp. 2122-2133.
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