Role of NADPH oxidase in retinal vascular inflammation

Mohamed Al-Shabrawey, Modesto Antonio Rojas, Tammy Sanders, Ali Behzadian, Azza El-Remessy, Manuela Bartoli, Abdul Kader Parpia, Gregory I Liou, Ruth B Caldwell

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

PURPOSE. In another study, it was demonstrated that NADPH oxidase-derived reactive oxygen species (ROS) are important for ischemia-induced increases in vascular endothelial growth factor (VEGF) and retinal neovascularization. Diabetes-induced increases in retinal ROS, VEGF expression, and vascular permeability are accompanied by increases in the NADPH oxidase catalytic subunit NOX2 within the retinal vessels. The goal of this study was to evaluate the potential role of NOX2 and NADPH oxidase activity in the development of retinal vascular inflammation. METHODS. Studies were performed in wild-type mice, mice lacking NOX2, and mice treated with the NADPH oxidase inhibitor apocynin in models of endotoxemia and streptozotocin-induced diabetes. Intracellular adhesion molecule (ICAM)-1 expression was determined by Western blot analysis. Leukocyte adhesion was assessed by labeling adherent leukocytes with concanavalin A. Vascular permeability was assessed by extravasation of FITC-conjugated albumin. ROS production was determined by dichlorofluorescein imaging. RESULTS. Both endotoxemia- and diabetes-induced increases in ICAM-1 expression and leukostasis were significantly inhibited by deletion of NOX2, indicating that this enzyme is critically involved in both conditions. Moreover, apocynin treatment and deletion of NOX2 were equally effective in preventing diabetes-induced increases in ICAM-1, leukostasis, and breakdown of the blood-retinal barrier, suggesting that NOX2 is primarily responsible for these early signs of diabetic retinopathy. CONCLUSIONS. These data suggest that NOX2 activity has a primary role in retinal vascular inflammation during acute and chronic conditions associated with retinal vascular inflammatory reactions. Targeting this enzyme could be a novel therapeutic strategy for treatment of the retinopathies associated with vascular inflammation.

Original languageEnglish (US)
Pages (from-to)3239-3244
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number7
DOIs
StatePublished - Jul 1 2008

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Retinal Vessels
NADPH Oxidase
Leukostasis
Inflammation
Reactive Oxygen Species
Endotoxemia
Capillary Permeability
Vascular Endothelial Growth Factor A
Leukocytes
Blood-Retinal Barrier
Retinal Neovascularization
Experimental Diabetes Mellitus
Diabetic Retinopathy
Enzymes
Concanavalin A
Blood Vessels
Catalytic Domain
Ischemia
Western Blotting
acetovanillone

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Role of NADPH oxidase in retinal vascular inflammation. / Al-Shabrawey, Mohamed; Rojas, Modesto Antonio; Sanders, Tammy; Behzadian, Ali; El-Remessy, Azza; Bartoli, Manuela; Parpia, Abdul Kader; Liou, Gregory I; Caldwell, Ruth B.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 7, 01.07.2008, p. 3239-3244.

Research output: Contribution to journalArticle

Al-Shabrawey, Mohamed ; Rojas, Modesto Antonio ; Sanders, Tammy ; Behzadian, Ali ; El-Remessy, Azza ; Bartoli, Manuela ; Parpia, Abdul Kader ; Liou, Gregory I ; Caldwell, Ruth B. / Role of NADPH oxidase in retinal vascular inflammation. In: Investigative Ophthalmology and Visual Science. 2008 ; Vol. 49, No. 7. pp. 3239-3244.
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T1 - Role of NADPH oxidase in retinal vascular inflammation

AU - Al-Shabrawey, Mohamed

AU - Rojas, Modesto Antonio

AU - Sanders, Tammy

AU - Behzadian, Ali

AU - El-Remessy, Azza

AU - Bartoli, Manuela

AU - Parpia, Abdul Kader

AU - Liou, Gregory I

AU - Caldwell, Ruth B

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N2 - PURPOSE. In another study, it was demonstrated that NADPH oxidase-derived reactive oxygen species (ROS) are important for ischemia-induced increases in vascular endothelial growth factor (VEGF) and retinal neovascularization. Diabetes-induced increases in retinal ROS, VEGF expression, and vascular permeability are accompanied by increases in the NADPH oxidase catalytic subunit NOX2 within the retinal vessels. The goal of this study was to evaluate the potential role of NOX2 and NADPH oxidase activity in the development of retinal vascular inflammation. METHODS. Studies were performed in wild-type mice, mice lacking NOX2, and mice treated with the NADPH oxidase inhibitor apocynin in models of endotoxemia and streptozotocin-induced diabetes. Intracellular adhesion molecule (ICAM)-1 expression was determined by Western blot analysis. Leukocyte adhesion was assessed by labeling adherent leukocytes with concanavalin A. Vascular permeability was assessed by extravasation of FITC-conjugated albumin. ROS production was determined by dichlorofluorescein imaging. RESULTS. Both endotoxemia- and diabetes-induced increases in ICAM-1 expression and leukostasis were significantly inhibited by deletion of NOX2, indicating that this enzyme is critically involved in both conditions. Moreover, apocynin treatment and deletion of NOX2 were equally effective in preventing diabetes-induced increases in ICAM-1, leukostasis, and breakdown of the blood-retinal barrier, suggesting that NOX2 is primarily responsible for these early signs of diabetic retinopathy. CONCLUSIONS. These data suggest that NOX2 activity has a primary role in retinal vascular inflammation during acute and chronic conditions associated with retinal vascular inflammatory reactions. Targeting this enzyme could be a novel therapeutic strategy for treatment of the retinopathies associated with vascular inflammation.

AB - PURPOSE. In another study, it was demonstrated that NADPH oxidase-derived reactive oxygen species (ROS) are important for ischemia-induced increases in vascular endothelial growth factor (VEGF) and retinal neovascularization. Diabetes-induced increases in retinal ROS, VEGF expression, and vascular permeability are accompanied by increases in the NADPH oxidase catalytic subunit NOX2 within the retinal vessels. The goal of this study was to evaluate the potential role of NOX2 and NADPH oxidase activity in the development of retinal vascular inflammation. METHODS. Studies were performed in wild-type mice, mice lacking NOX2, and mice treated with the NADPH oxidase inhibitor apocynin in models of endotoxemia and streptozotocin-induced diabetes. Intracellular adhesion molecule (ICAM)-1 expression was determined by Western blot analysis. Leukocyte adhesion was assessed by labeling adherent leukocytes with concanavalin A. Vascular permeability was assessed by extravasation of FITC-conjugated albumin. ROS production was determined by dichlorofluorescein imaging. RESULTS. Both endotoxemia- and diabetes-induced increases in ICAM-1 expression and leukostasis were significantly inhibited by deletion of NOX2, indicating that this enzyme is critically involved in both conditions. Moreover, apocynin treatment and deletion of NOX2 were equally effective in preventing diabetes-induced increases in ICAM-1, leukostasis, and breakdown of the blood-retinal barrier, suggesting that NOX2 is primarily responsible for these early signs of diabetic retinopathy. CONCLUSIONS. These data suggest that NOX2 activity has a primary role in retinal vascular inflammation during acute and chronic conditions associated with retinal vascular inflammatory reactions. Targeting this enzyme could be a novel therapeutic strategy for treatment of the retinopathies associated with vascular inflammation.

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