Role of ONO-3144, a new cardioplegic agent, in the reoxygenation injury in the anoxic myocardium

Hiroshi Kobayashi, Muhammad Ashraf, Kaoru Kobayashi, Meyian Rahamathulia, Arnold Schwartz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have investigated the effect of ONO-3144 (2-aminomethyl-4-tert-butyl-6-propionylphenol), which facilitates the conversion of prostaglandin G2 to H2 and acts as a scavenger of free radicals, on the reoxygenation injury in the anoxic heart. Rat hearts were perfused retrogradely with Krebs-Henseleit (KH) medium for 30 min (n = 8) in Group I. In Group II, the hearts which were perfused with anoxic KH medium for 40 min were reoxygenated for 30 min (n = 8). Group III hearts were similar to those in Group II except that 4 mg ONO-3144/liter was added to both anoxic and reoxygenation media (n = 8). Coronary effluent was collected for creatine kinase (CK) loss. Four rats hearts in each group were fixed for electron microscopic study and the remaining hearts were frozen in liquid nitrogen for measurement of adenosine triphosphate (ATP). A six-fold increase in CK leakage, observed after reoxygenation of anoxic heart, was prevented by ONO-3144. Tissue ATP was reduced from 22.2 ± 0.9 jUmol/g dry weight (Group I) to 5.5 ± 1.1 ³mol/g dry weight (Group II). A significant amount of ATP (9.05 ± 1.22 jUmol/g dry weight) was preserved in the treated Group III. The number of normal cells obtained by morphometrical analysis increased significantly from 56.7 ±7.8% (Group II) to 86.2 ± 1.0% (Group III) and moderately injured cells were reduced to 3% in Group III as compared to 16% in the untreated Group I. Injury to the severely injured cells was not prevented by the drug treatment. At electron microscopic level, the cellular membranes, mitochondria and glycogen deposits were well preserved in Group III. Thus, ONO-3144 treatment provides a protection against reoxygenation injury in the anoxic myocardium by scavenging. OH or other closely related species of free radicals. Therefore, free radicals generated through the conversion of prostaglandin G2 to H2 might play an important role in the reoxygenation injury of the anoxic myocardium.

Original languageEnglish (US)
Pages (from-to)421-430
Number of pages10
JournalJAPANESE CIRCULATION JOURNAL
Volume51
Issue number4
DOIs
StatePublished - Jan 1 1987

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Myocardium
Wounds and Injuries
Adenosine Triphosphate
Creatine Kinase
Weights and Measures
Free Radicals
Electrons
Free Radical Scavengers
2-aminomethyl-4-tert-butyl-6-propionylphenol
Glycogen
Mitochondria
Nitrogen
Cell Count
Membranes
Pharmaceutical Preparations

Keywords

  • Anoxia
  • Electron microscopy
  • Oxygen free radicals
  • Reoxygenation
  • Reperfusion injury

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Role of ONO-3144, a new cardioplegic agent, in the reoxygenation injury in the anoxic myocardium. / Kobayashi, Hiroshi; Ashraf, Muhammad; Kobayashi, Kaoru; Rahamathulia, Meyian; Schwartz, Arnold.

In: JAPANESE CIRCULATION JOURNAL, Vol. 51, No. 4, 01.01.1987, p. 421-430.

Research output: Contribution to journalArticle

Kobayashi, Hiroshi ; Ashraf, Muhammad ; Kobayashi, Kaoru ; Rahamathulia, Meyian ; Schwartz, Arnold. / Role of ONO-3144, a new cardioplegic agent, in the reoxygenation injury in the anoxic myocardium. In: JAPANESE CIRCULATION JOURNAL. 1987 ; Vol. 51, No. 4. pp. 421-430.
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N2 - We have investigated the effect of ONO-3144 (2-aminomethyl-4-tert-butyl-6-propionylphenol), which facilitates the conversion of prostaglandin G2 to H2 and acts as a scavenger of free radicals, on the reoxygenation injury in the anoxic heart. Rat hearts were perfused retrogradely with Krebs-Henseleit (KH) medium for 30 min (n = 8) in Group I. In Group II, the hearts which were perfused with anoxic KH medium for 40 min were reoxygenated for 30 min (n = 8). Group III hearts were similar to those in Group II except that 4 mg ONO-3144/liter was added to both anoxic and reoxygenation media (n = 8). Coronary effluent was collected for creatine kinase (CK) loss. Four rats hearts in each group were fixed for electron microscopic study and the remaining hearts were frozen in liquid nitrogen for measurement of adenosine triphosphate (ATP). A six-fold increase in CK leakage, observed after reoxygenation of anoxic heart, was prevented by ONO-3144. Tissue ATP was reduced from 22.2 ± 0.9 jUmol/g dry weight (Group I) to 5.5 ± 1.1 ³mol/g dry weight (Group II). A significant amount of ATP (9.05 ± 1.22 jUmol/g dry weight) was preserved in the treated Group III. The number of normal cells obtained by morphometrical analysis increased significantly from 56.7 ±7.8% (Group II) to 86.2 ± 1.0% (Group III) and moderately injured cells were reduced to 3% in Group III as compared to 16% in the untreated Group I. Injury to the severely injured cells was not prevented by the drug treatment. At electron microscopic level, the cellular membranes, mitochondria and glycogen deposits were well preserved in Group III. Thus, ONO-3144 treatment provides a protection against reoxygenation injury in the anoxic myocardium by scavenging. OH or other closely related species of free radicals. Therefore, free radicals generated through the conversion of prostaglandin G2 to H2 might play an important role in the reoxygenation injury of the anoxic myocardium.

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KW - Electron microscopy

KW - Oxygen free radicals

KW - Reoxygenation

KW - Reperfusion injury

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