Role of plasminogen activators during healing after uterine serosal lesioning in the rat

Ujjwal Kumar Rout, Michael Peter Diamond

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalFertility and Sterility
Volume79
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Plasminogen Activators
Reverse Transcriptase Polymerase Chain Reaction
Blood Vessels
Tissue Adhesions
Urokinase-Type Plasminogen Activator
Ambulatory Surgical Procedures
Up-Regulation
Outcome Assessment (Health Care)
Staining and Labeling
Wounds and Injuries

Keywords

  • Adhesion
  • Angiogenesis
  • Immunocytochemistry
  • Rat
  • Reverse transcriptase-PCR
  • t-PA
  • u-PA

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Role of plasminogen activators during healing after uterine serosal lesioning in the rat. / Rout, Ujjwal Kumar; Diamond, Michael Peter.

In: Fertility and Sterility, Vol. 79, No. 1, 01.01.2003, p. 138-145.

Research output: Contribution to journalArticle

@article{edc3a33f1a3f43aa827ec481b7ee4e41,
title = "Role of plasminogen activators during healing after uterine serosal lesioning in the rat",
abstract = "Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.",
keywords = "Adhesion, Angiogenesis, Immunocytochemistry, Rat, Reverse transcriptase-PCR, t-PA, u-PA",
author = "Rout, {Ujjwal Kumar} and Diamond, {Michael Peter}",
year = "2003",
month = "1",
day = "1",
doi = "10.1016/S0015-0282(02)04569-7",
language = "English (US)",
volume = "79",
pages = "138--145",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Role of plasminogen activators during healing after uterine serosal lesioning in the rat

AU - Rout, Ujjwal Kumar

AU - Diamond, Michael Peter

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.

AB - Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.

KW - Adhesion

KW - Angiogenesis

KW - Immunocytochemistry

KW - Rat

KW - Reverse transcriptase-PCR

KW - t-PA

KW - u-PA

UR - http://www.scopus.com/inward/record.url?scp=0037228338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037228338&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(02)04569-7

DO - 10.1016/S0015-0282(02)04569-7

M3 - Article

VL - 79

SP - 138

EP - 145

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 1

ER -