Role of plasminogen activators during healing after uterine serosal lesioning in the rat

Ujjwal Kumar Rout, Michael P. Diamond

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalFertility and sterility
Volume79
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Adhesion
  • Angiogenesis
  • Immunocytochemistry
  • Rat
  • Reverse transcriptase-PCR
  • t-PA
  • u-PA

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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