Role of selenium-containing proteins in T-cell and macrophage function

Bradley A. Carlson, Min Hyuk Yoo, Rajeev Kumar Shrimali, Robert Irons, Vadim N. Gladyshev, Dolph L. Hatfield, Jin Mo Park

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Selenium (Se) has been known for many years to have played a role in boosting the immune function, but the manner in which this element acts at the molecular level in host defence and inflammatory diseases is poorly understood. To elucidate the role of Se-containing proteins in the immune function, we knocked out the expression of this protein class in T-cells or macrophages of mice by targeting the removal of the selenocysteine tRNA gene using loxP-Cre technology. Mice with selenoprotein-less T-cells manifested reduced pools of mature and functional T-cells in lymphoid tissues and an impairment in T-cell-dependent antibody responses. Furthermore, selenoprotein deficiency in T-cells led to an inability of these cells to suppress reactive oxygen species production, which in turn affected their ability to proliferate in response to T-cell receptor stimulation. Selenoprotein-less macrophages, on the other hand, manifested mostly normal inflammatory responses, but this deficiency resulted in an altered regulation in extracellular matrix-related gene expression and a diminished migration of macrophages in a protein gel matrix. These observations provided novel insights into the role of selenoproteins in the immune function and tissue homeostasis.

Original languageEnglish (US)
Pages (from-to)300-310
Number of pages11
JournalProceedings of the Nutrition Society
Volume69
Issue number3
DOIs
StatePublished - Aug 1 2010

Fingerprint

Selenoproteins
Selenium
Macrophages
T-Lymphocytes
Proteins
Lymphoid Tissue
T-Cell Antigen Receptor
Antibody Formation
Extracellular Matrix
Reactive Oxygen Species
Homeostasis
Gels
Technology
Gene Expression
Genes

Keywords

  • Macrophage
  • Selenium
  • Selenocysteine
  • T-cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Carlson, B. A., Yoo, M. H., Shrimali, R. K., Irons, R., Gladyshev, V. N., Hatfield, D. L., & Park, J. M. (2010). Role of selenium-containing proteins in T-cell and macrophage function. Proceedings of the Nutrition Society, 69(3), 300-310. https://doi.org/10.1017/S002966511000176X

Role of selenium-containing proteins in T-cell and macrophage function. / Carlson, Bradley A.; Yoo, Min Hyuk; Shrimali, Rajeev Kumar; Irons, Robert; Gladyshev, Vadim N.; Hatfield, Dolph L.; Park, Jin Mo.

In: Proceedings of the Nutrition Society, Vol. 69, No. 3, 01.08.2010, p. 300-310.

Research output: Contribution to journalArticle

Carlson, BA, Yoo, MH, Shrimali, RK, Irons, R, Gladyshev, VN, Hatfield, DL & Park, JM 2010, 'Role of selenium-containing proteins in T-cell and macrophage function', Proceedings of the Nutrition Society, vol. 69, no. 3, pp. 300-310. https://doi.org/10.1017/S002966511000176X
Carlson BA, Yoo MH, Shrimali RK, Irons R, Gladyshev VN, Hatfield DL et al. Role of selenium-containing proteins in T-cell and macrophage function. Proceedings of the Nutrition Society. 2010 Aug 1;69(3):300-310. https://doi.org/10.1017/S002966511000176X
Carlson, Bradley A. ; Yoo, Min Hyuk ; Shrimali, Rajeev Kumar ; Irons, Robert ; Gladyshev, Vadim N. ; Hatfield, Dolph L. ; Park, Jin Mo. / Role of selenium-containing proteins in T-cell and macrophage function. In: Proceedings of the Nutrition Society. 2010 ; Vol. 69, No. 3. pp. 300-310.
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