Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU

Simon Lattmann, Banabihari Giri, James P. Vaughn, Steven A. Akman, Yoshikuni Nagamine

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Under physiological conditions, guanine-rich sequences of DNA and RNA can adopt stable and atypical four-stranded helical structures called G-quadruplexes (G4). Such G4 structures have been shown to occur in vivo and to play a role in various processes such as transcription, translation and telomere maintenance. Owing to their high-thermodynamic stability, resolution of G4 structures in vivo requires specialized enzymes. RHAU is a human RNA helicase of the DEAH-box family that exhibits a unique ATP-dependent G4-resolvase activity with a high affinity and specificity for its substrate in vitro. How RHAU recognizes G4-RNAs has not yet been established. Here, we show that the amino-terminal region of RHAU is essential for RHAU to bind G4 structures and further identify within this region the evolutionary conserved RSM (RHAU-specific motif) domain as a major affinity and specificity determinant. G4-resolvase activity and strict RSM dependency are also observed with CG9323, the Drosophila orthologue of RHAU, in the amino terminal region of which the RSM is the only conserved motif. Thus, these results reveal a novel motif in RHAU protein that plays an important role in recognizing and resolving G4-RNA structures, properties unique to RHAU among many known RNA helicases.

Original languageEnglish (US)
Article numbergkq372
Pages (from-to)6219-6233
Number of pages15
JournalNucleic Acids Research
Volume38
Issue number18
DOIs
StatePublished - May 14 2010

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RNA Helicases
G-Quadruplexes
Recombinases
RNA
Telomere
Guanine
Substrate Specificity
Thermodynamics
Drosophila
Adenosine Triphosphate
Maintenance
Enzymes
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics

Cite this

Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. / Lattmann, Simon; Giri, Banabihari; Vaughn, James P.; Akman, Steven A.; Nagamine, Yoshikuni.

In: Nucleic Acids Research, Vol. 38, No. 18, gkq372, 14.05.2010, p. 6219-6233.

Research output: Contribution to journalArticle

Lattmann, Simon ; Giri, Banabihari ; Vaughn, James P. ; Akman, Steven A. ; Nagamine, Yoshikuni. / Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. In: Nucleic Acids Research. 2010 ; Vol. 38, No. 18. pp. 6219-6233.
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