Rutin protects the neural damage induced by transient focal ischemia in rats

Mohd Moshahid Khan, Ajmal Ahmad, Tauheed Ishrat, Gulrana Khuwaja, Pallavi Srivastawa, Mohammad Badruzzaman Khan, Syed Shadab Raza, Hayate Javed, Kumar Vaibhav, Andleeb Khan, Fakhrul Islam

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Free radical induced neural damage is implicated in cerebral ischemia-reperfusion (IR) injury and antioxidants are reported to have neuroprotective activity. The present study was designed to assess the neuroprotective role of rutin (Vitamin P), and mechanism of action. The middle cerebral artery (MCA) of an adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The administration of rutin (25 mg/kg bwt., orally) once daily for 21 days before middle cerebral artery occlusion (MCAO) showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. A significantly depleted activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) and content of glutathione (GSH) in MCAO group were protected significantly in MCAO group pretreated with rutin. Conversely, the elevated level of thiobarbituric acid reactive species (TBARS), H2O2 and protein carbonyl (PC) in MCAO group was attenuated significantly in rutin-pretreated group when compared with MCAO group. These results indicate that rutin attenuates ischemic neural apoptosis by reducing the expression of p53, preventing morphological changes and increasing endogenous antioxidant enzymatic activities. Thus, rutin treatment may represent a novel approach in lowering the risk or improving the function of ischemia-reperfusion brain injury-related disorders.

Original languageEnglish (US)
Pages (from-to)123-135
Number of pages13
JournalBrain Research
Volume1292
DOIs
StatePublished - Sep 25 2009

Keywords

  • Apoptosis
  • Brain infarct
  • Ischemia
  • Neurobehavior
  • Oxidative stress
  • Rutin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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