S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2

Sean S. Shaw, Ann Marie Schmidt, Amy K. Banes, Xiaodan Wang, David M. Stern, Mario B Marrero

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Angiotensin II (Ang II), a vasoactive peptide that is also considered a growth factor, has been implicated in both normal and diabetic cellular proliferation. We recently found that activation of janus kinase 2 (JAK2) is essential for the Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) and that high glucose augments Ang II-induced proliferation of VSMCs by increasing signal transduction through activation of JAK2. Here, we demonstrate that S100B, a ligand for the receptor of advanced glycation end products (RAGEs), augmented both Ang II-induced tyrosine phosphorylation of JAK2 and cell proliferation in VSMCs in a receptor-dependent manner. We also found that S100B-RAGE interaction triggered intracellular generation of reactive oxygen species (ROS), VSMC proliferation, and JAK2 tyrosine phosphorylation via activation of phospholipase D (PLD)2. These results provide direct evidence for linkages between PLD2, ROS production, and S100B-RAGE-induced enhancement of Ang II-induced cell proliferation and activation of JAK2 in VSMCs.

Original languageEnglish (US)
Pages (from-to)2381-2388
Number of pages8
JournalDiabetes
Volume52
Issue number9
DOIs
StatePublished - Sep 1 2003

Fingerprint

Janus Kinase 2
Vascular Smooth Muscle
Angiotensin II
Smooth Muscle Myocytes
Cell Proliferation
Reactive Oxygen Species
Phosphorylation
Phospholipase D
Tyrosine
Signal Transduction
Intercellular Signaling Peptides and Proteins
Advanced Glycosylation End Product-Specific Receptor
Ligands
Glucose
Peptides

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2. / Shaw, Sean S.; Schmidt, Ann Marie; Banes, Amy K.; Wang, Xiaodan; Stern, David M.; Marrero, Mario B.

In: Diabetes, Vol. 52, No. 9, 01.09.2003, p. 2381-2388.

Research output: Contribution to journalArticle

Shaw, Sean S. ; Schmidt, Ann Marie ; Banes, Amy K. ; Wang, Xiaodan ; Stern, David M. ; Marrero, Mario B. / S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2. In: Diabetes. 2003 ; Vol. 52, No. 9. pp. 2381-2388.
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